Esfandani-Bozchaloyi Somayeh, Zaman Wajid
Faculty Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
Microsc Res Tech. 2018 Dec;81(12):1520-1532. doi: 10.1002/jemt.23159. Epub 2018 Nov 19.
Macro and micromorphology of different parts that is, sepals, petals, flowers, indumentum, and leaf shape of 22 Geranium L. taxa from Iran were studied. These taxa representing the eight sections of the genus as sect. Dissecta, sect. Geranium, and sect. Tuberosa (of subgen. Geranium); sect. Batrachioidea, sect. Divaricata, sect. Lucida, sect. Ruberta and sect. Trilopha (of subgen. Robertium), that were collected from different geographical habitats of Iran. The biometric study involved 27 quantitative and 33 qualitative characters which were studied by use of stereomicroscopy and scanning electron microscopy (SEM). Statistical analyses were done by use of PAST software. Principal Component Analysis defined the most variable characters. Results showed that the diagnostic features were; length, width, apex of sepals, petals, trichomes type in pedicles and sepals along with their density, the shape length and width of leaves. In general, the present study revealed that the species could be differentiated by macro and micro-morphological characters. Taxa of two subgenera were clearly separated based on selected characters. These characters were found useful for the taxonomic identification and species delimitation in almost all taxa studied at the sub generic level, although some species of section Geranium and section Dissecta are mixed and nested in both subgenea.
对来自伊朗的22个老鹳草属分类群不同部位(即萼片、花瓣、花朵、毛被和叶形)的宏观和微观形态进行了研究。这些分类群代表了该属的八个组,分别是组Dissecta、组Geranium和组Tuberosa(天竺葵亚属);组Batrachioidea、组Divaricata、组Lucida、组Ruberta和组Trilopha(罗伯蒂亚亚属),它们采自伊朗不同的地理栖息地。生物特征研究涉及27个数量性状和33个质量性状,通过体视显微镜和扫描电子显微镜(SEM)进行研究。使用PAST软件进行统计分析。主成分分析确定了最具变异性的性状。结果表明,诊断特征包括:萼片、花瓣的长度、宽度、顶端,花梗和萼片中毛状体的类型及其密度,叶片的形状、长度和宽度。总体而言,本研究表明,这些物种可以通过宏观和微观形态特征加以区分。基于选定的性状,两个亚属的分类群被明显区分开来。这些性状对于几乎所有在亚属水平研究的分类群的分类鉴定和物种界定都很有用,尽管天竺葵组和Dissecta组的一些物种在两个亚属中相互混杂和嵌套。