Khan Raees, Ahmad Mushtaq, Zafar Muhammad, Ullah Asad
Department of Plant Sciences, Quaid e Azam University Islamabad, Pakistan.
Centre of Plant Biodiversity, University of Peshawar, Khyber Pakhtun khwa, Pakistan.
Microsc Res Tech. 2017 Oct;80(10):1123-1140. doi: 10.1002/jemt.22909. Epub 2017 Jul 12.
SEM and light microscopy are of special interest for biologist to observe various features of the living bodies. In the current study we observed the foliar epidermal micro-morphological characters of 44 grass species using SEM and Light microscopy to assess their taxonomic utility for taxonomists in the identification process. The aim of this study is to use the foliar epidermal structural variations in both upper and lower surfaces for identification of grasses. Significant diversity was observed in both qualitative and quantitative characters using SEM and Light microscopy. Variations were observed in stomatal number, size, guard cells shape, silica bodies, macro-hairs, micro-hairs, epidermal cell number, subsidiary cells, prickles, hooks, papillae, and short and long cells. A taxonomic key is prepared using these variations for the identification of grass species. Based on these findings, SEM and Light microscopy of foliar epidermal features can be of special interest for taxonomists in the identification of complex grass taxa.
扫描电子显微镜（SEM）和光学显微镜对生物学家观察生物体的各种特征具有特殊意义。在本研究中，我们使用扫描电子显微镜和光学显微镜观察了44种草种的叶表皮微观形态特征，以评估其在分类过程中对分类学家的分类学效用。本研究的目的是利用上下叶表皮结构的变化来鉴定草种。使用扫描电子显微镜和光学显微镜观察到，在定性和定量特征方面都存在显著差异。观察到气孔数量、大小、保卫细胞形状、硅质体、大毛、微毛、表皮细胞数量、副卫细胞、皮刺、钩、乳头状突起以及长短细胞等方面的变化。利用这些变化编制了一个分类检索表，用于草种的鉴定。基于这些发现，叶表皮特征的扫描电子显微镜和光学显微镜观察对分类学家鉴定复杂的草类分类群可能具有特殊意义。
