Kantor Mihail, Levi Amnon, Thies Judith, Guner Nihat, Kantor Camelia, Parnham Stuart, Boroujerdi Arezue
Claflin University, 898 Goff Ave., Orangeburg, SC 29115 ; USDA, ARS, U.S. Vegetable Laboratory, 2700 Savannah Highway, Charleston, SC 29414.
USDA, ARS, U.S. Vegetable Laboratory, 2700 Savannah Highway, Charleston, SC 29414.
J Nematol. 2018;50(3):303-316. doi: 10.21307/jofnem-2018-030.
Citrullus amarus ( CA ) (previously known as Citrullus lanatus var. citroides ) accessions collected in southern Africa are known to have resistance to root-knot nematodes (RKN) and are suitable rootstocks for grafted watermelon. The objective of this study was to conduct a comparative metabolomics analysis and identify unique metabolites in roots of CA accessions versus roots of watermelon cultivars ( Citrullus lanatus (Thunb.) Matsum. and Nakai var. lanatus; CL ). Nuclear magnetic resonance (NMR) technology and principal component analysis (PCA) were used to analyze and compare metabolic profiles of seven CA accessions resistant to RKN along with two RKN-susceptible watermelon cultivars (Charleston Gray and Crimson Sweet). Calculation of the Mahalanobis distance revealed that the CA United States Plant Introduction (PI) 189225 (Line number 1832) and PI 482324 (1849) have the most distinct metabolic profiles compared with the watermelon cultivars Charleston Gray and Crimson Sweet, respectively. Several amino acids identified in the CA accessions were reported in previous studies to have a nematicidal effect. The results in this study indicate that roots of watermelon accessions collected in the wild are rich in metabolic compounds. These metabolic compounds may have been diminished in watermelon cultivars as a consequence of many years of cultivation and selection for desirable fruit qualities.
在非洲南部收集的苦西瓜（CA）（以前称为西瓜变种 citroides）种质已知对根结线虫（RKN）具有抗性，是嫁接西瓜的合适砧木。本研究的目的是进行比较代谢组学分析，并鉴定苦西瓜种质根系与西瓜品种（西瓜（Thunb.）Matsum.和 Nakai var. lanatus；CL）根系中的独特代谢物。利用核磁共振（NMR）技术和主成分分析（PCA）分析和比较了七个抗根结线虫的苦西瓜种质以及两个感根结线虫的西瓜品种（查尔斯顿灰和绯红甜心）的代谢谱。马氏距离计算表明，与西瓜品种查尔斯顿灰和绯红甜心相比，苦西瓜美国植物引种编号（PI）189225（品系编号1832）和PI 482324（1849）具有最独特的代谢谱。先前的研究报道，在苦西瓜种质中鉴定出的几种氨基酸具有杀线虫作用。本研究结果表明，野生西瓜种质的根系富含代谢化合物。由于多年来对理想果实品质的栽培和选择，这些代谢化合物在西瓜品种中可能已经减少。
