Zhao Lixiang, Dong Junjian, Sun Chengfei, Tian Yuanyuan, Hu Jie, Ye Xing
a Key Laboratory of Tropical & Subtropical Fisheries Resource Application & Cultivation, Ministry of Agriculture , Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences , Guangzhou , China.
b College of Fisheries and Life Science , Shanghai Ocean University , Shanghai , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2019 Mar;30(2):234-248. doi: 10.1080/24701394.2018.1482283. Epub 2018 Nov 19.
Perciformes is the largest order of fishes and vertebrates. Sooty grunter (Hephaestus fuliginosus) is an economic fish species in the Terapontidae family of Percoidei, a suborder within Perciformes. To conduct molecular-level analysis of the phylogenetic relationships between sooty grunter and major freshwater fishes in Percoidei, we analysed the entire sooty grunter mitochondrial genome sequence and obtained the mitochondrial genome information of 19 fishes from Terapontidae, Serranidae, and Centrarchidae families in Percoidei from GenBank. The complete length of the sooty grunter mitochondrial genome was 16,770 bp; it encoded 13 proteins, 2 rRNAs, 22 tRNAs, and a displacement loop (D-loop). Other than ND6 and eight tRNA genes that are encoded by the light strand, the majority of genes are encoded by the heavy strand. The sequence and distribution of sooty grunter mitochondrial-encoded genes and non-coding segment were similar to those of most vertebrates. The results of neighbour joining, maximum parsimony, and Bayesian inference analyses of the complete mitochondrial genome and six genes, including cytochrome oxidase I, cytochrome B, 12S rRNA, ND2, ND4, and ND5, were consistent. In the phylogenetic trees, fishes in Terapontidae and Centrarchidae formed monophyletic clades, whereas those in Serranidae were divided into two clades, each containing Lateolabrax and Siniperca species. Among the three freshwater fish species in Terapontidae, the freshwater Terapontidae were more closely related to jade perch than with silver perch, suggesting that freshwater Terapontidae fishes originate from marine fishes. In addition, the phylogenetic results indicated that Micropterus salmoides salmoides and Micropterus salmoides floridanus in Centrarchidae should be designated as two independent species, and Siniperca in Serranidae should be considered an independent family. The sooty grunter mitochondrial genome sequence obtained in this study could be used to conduct population genetic diversity and germplasm resource studies. Furthermore, the phylogenetic analysis results of freshwater fishes in Percoidei could provide a molecular basis for cross-breeding.
鲈形目是鱼类和脊椎动物中最大的目。乌鳍石鲈（Hephaestus fuliginosus）是鲈形目鲈亚目石鲈科的一种经济鱼类。为了在分子水平上分析乌鳍石鲈与鲈亚目主要淡水鱼类之间的系统发育关系，我们分析了乌鳍石鲈的完整线粒体基因组序列，并从GenBank获取了鲈亚目石鲈科、鮨科和太阳鱼科19种鱼类的线粒体基因组信息。乌鳍石鲈线粒体基因组全长16770 bp；它编码13种蛋白质、2种rRNA、22种tRNA和一个位移环（D-loop）。除了由轻链编码的ND6和8个tRNA基因外，大多数基因由重链编码。乌鳍石鲈线粒体编码基因和非编码区的序列及分布与大多数脊椎动物相似。对完整线粒体基因组以及包括细胞色素氧化酶I、细胞色素B、12S rRNA、ND2、ND4和ND5在内的6个基因进行邻接法、最大简约法和贝叶斯推断分析的结果是一致的。在系统发育树中，石鲈科和太阳鱼科的鱼类形成单系类群，而鮨科的鱼类分为两个类群，每个类群包含花鲈属和鳜属物种。在石鲈科的三种淡水鱼中，淡水石鲈科与宝石鲈的亲缘关系比与银鲈更近，这表明淡水石鲈科鱼类起源于海洋鱼类。此外，系统发育结果表明，太阳鱼科的大口黑鲈指名亚种和大口黑鲈佛罗里达亚种应被指定为两个独立的物种，鮨科的鳜属应被视为一个独立的科。本研究获得的乌鳍石鲈线粒体基因组序列可用于进行群体遗传多样性和种质资源研究。此外，鲈亚目淡水鱼类的系统发育分析结果可为杂交育种提供分子基础。
