Department of Nuclear Medicine, the First Affiliated Hospital of Soochow University, Suzhou 215006, China.
State Key Laboratory of Radiation Medicine & Protection, School for Radiological & Interdisciplinary Sciences (RAD-X) & Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou 215123, China.
Nanomedicine (Lond). 2019 Jan;14(1):5-17. doi: 10.2217/nnm-2018-0219. Epub 2018 Nov 19.
In the present study, we aimed to characterize the tumor-targeting properties of ultra-small iron oxide nanoparticles (IONPs) as multimodality imaging contrast agent.
The dimeric cRGD peptides [cyclic(Cys-Arg-Gly-Asp-dSer-Cys)-Tyr-dSer-Lys-Tyr-cyclic(Cys-Arg-Gly-Asp-dSer-Cys)], which specifically targeted integrin-αβ receptor highly overexpressed in tumor vasculature and tumor cells, were covalently conjugated onto the surface of ultra-small IONPs followed by the labeling of nuclide I through the chloramine-T method to afford the desired I-(cRGD)-IONPs nanoprobe.I-(cRGD)-IONPs were injected into tumor-bearing mice for magnetic resonance (MR) and single photon emission computed tomography (SPECT) multi-modality imaging of tumors.
The prepared IONPs demonstrated were very useful for T1/T2 and SPECT imaging of tumors in vivo, exhibiting a high tumor uptake of a clinically useful target-to-background ratio in a short time.
We successfully developed a novel integrin-αβ receptor-targeted ultra-small IONPs, which could be successfully used as T1-T2-MRI/SPECT contrast agents for high-resolution and high-sensitivity of tumor imaging in vivo.
在本研究中，我们旨在表征超小氧化铁纳米粒子（IONPs）作为多模态成像造影剂的肿瘤靶向特性。
二聚体 cRGD 肽 [环（Cys-Arg-Gly-Asp-dSer-Cys）-Tyr-dSer-Lys-Tyr-环（Cys-Arg-Gly-Asp-dSer-Cys）]，特异性靶向整合素-αβ受体，该受体在肿瘤血管和肿瘤细胞中过度表达，通过氯胺-T 法将其共价连接到超小 IONPs 的表面，然后通过氯胺-T 法对核素 I 进行标记，得到所需的 I-（cRGD）-IONPs 纳米探针。将 I-（cRGD）-IONPs 注射到荷瘤小鼠中，进行肿瘤的磁共振（MR）和单光子发射计算机断层扫描（SPECT）多模态成像。
所制备的 IONPs 非常适用于体内肿瘤的 T1/T2 和 SPECT 成像，在短时间内表现出高肿瘤摄取和具有临床应用价值的靶背比。
我们成功开发了一种新型整合素-αβ 受体靶向超小 IONPs，可成功用作 T1-T2-MRI/SPECT 造影剂，用于体内肿瘤的高分辨率和高灵敏度成像。
