Silchenkoa Alexandra S, Kalinovsky Anatoly I, Avilov Sergey A, Andryjaschenko Pelageya V, Dmitrenok Pavel S, Kalinin Vladimir I, Martyyas Ekaterina A, Minin Kirill V
Nat Prod Commun. 2016 Jul;11(7):939-945.
Four hew triterpene glycosides, fallaxosides C, (1), C2 (2), D, (3) and D2 (4) along with the known cucumarioside A3-2 (5) and koreoside A (6) have-been isolated from the sea cucumber Cucumaria fallax (Cucumariidae, Dendrochirotida). Structures of the glycosides have been elucidated by 2D NMR spectroscopy and mass spectrometry. All of the glycosides are rare non-holostane derivatives having shortened side chains and contain pentasaccharide carbohydrate moieties with two or three sulfate groups. Structures of these triterpene glycosides and their comparison with those earlier isolated from Cucuniaria spp. and Pseudocnus dubiosus leoninus allow us to suggest that the present assignment of C. fallax to the genus Pseudocnus is not correct, and this species.should be assigned to the genus Cucumaria. Cytotoxic activity of glycosides 1-5 against the ascite form of mouse Ehrlich carcinoma cells and mouse spleen lymphocytes and hemolytic activity against mouse erythrocytes have been studied. The glycosides were expectedly not active in all the tests due to the absence of an 18(20)-lactone in their aglycones and the presence of several sulfate groups. There was one exception, cucumarioside A₃-2 (5), which. demonstrated a weak cytotoxicity against lymphocytes and moderate hemolytic activity.
从海参Cucumaria fallax（瓜参科，枝手目）中分离出了四种新的三萜糖苷，即fallaxosides C（1）、C2（2）、D（3）和D2（4），以及已知的海参苷A3-2（5）和朝鲜参苷A（6）。糖苷的结构已通过二维核磁共振光谱和质谱进行了阐明。所有糖苷都是罕见的非全氢甾烷衍生物，具有缩短的侧链，并且含有带有两个或三个硫酸基团的五糖碳水化合物部分。这些三萜糖苷的结构及其与先前从Cucuniaria spp.和Pseudocnus dubiosus leoninus中分离出的糖苷的比较使我们认为，目前将C. fallax归为Pseudocnus属是不正确的，该物种应归为Cucumaria属。研究了糖苷1-5对小鼠艾氏腹水癌细胞和小鼠脾脏淋巴细胞的细胞毒性活性以及对小鼠红细胞的溶血活性。由于苷元中不存在18(20)-内酯且存在多个硫酸基团，预计这些糖苷在所有测试中均无活性。有一个例外，即海参苷A₃-2（5），它对淋巴细胞表现出弱细胞毒性和中等溶血活性。
