Whistler T, Swanepoel R
Department of Virology, University of the Witwatersra, South Africa.
J Gen Virol. 1988 Sep;69 ( Pt 9):2221-7. doi: 10.1099/0022-1317-69-9-2221.
Twelve Palyam serogroup orbiviruses isolated in Zimbabwe from aborted cattle foetuses, plus one isolated from the visceral organs of a cow and another from vulture faeces, were examined in comparison with known members of the serogroup by complement fixation, indirect immunofluorescence, fluorescent focus reduction neutralization tests and PAGE of the segmented, dsRNA genomes. The viruses were indistinguishable from known members of the serogroup by complement fixation and indirect immunofluorescence, but two novel viruses, for which the names Gweru and Marondera are proposed, and two previously described viruses, Nyabira and Abadina, were identified by neutralization tests. The dsRNA profiles of Abadina serotype isolates differed from that of the Abadina prototype virus, indicating that different electropherotypes may occur within serotypes.
对从津巴布韦流产牛胎儿中分离出的12株帕利亚姆血清群环状病毒，以及从一头母牛内脏器官中分离出的1株和从秃鹫粪便中分离出的1株，与该血清群已知成员进行了比较，采用补体结合试验、间接免疫荧光试验、荧光灶减少中和试验以及对分段双链RNA基因组进行聚丙烯酰胺凝胶电泳分析。这些病毒通过补体结合试验和间接免疫荧光试验与该血清群已知成员无法区分，但通过中和试验鉴定出两种新型病毒（分别命名为圭鲁病毒和马隆德拉病毒）以及两种先前描述过的病毒（尼亚比拉病毒和阿巴迪纳病毒）。阿巴迪纳血清型分离株的双链RNA图谱与阿巴迪纳原型病毒不同，表明血清型内可能存在不同的电泳型。
