Knudson D L, Tesh R B, Main A J, St George T D, Digoutte J P
Intervirology. 1984;22(1):41-9. doi: 10.1159/000149532.
31 Palyam serogroup viruses were examined by complement-fixation and plaque-reduction neutralization tests and by PAGE of the segmented, double-stranded (ds) RNA genome. Although the viruses were indistinguishable by complement-fixation tests, 10 distinct virus serotypes were identified by plaque-reduction neutralization methods. Palyam group viruses which were distinct by the neutralization test had unique dsRNA profiles, whereas those agents which were indistinct by the neutralization test had identical dsRNA profiles. 20 isolations of 3 Palyam serotypes were made from bovines and Culicoides midges in Australia over a 9-year period. When the genome of these isolates was examined electrophoretically, the dsRNA profiles of virus isolates within a given serotype were identical.
通过补体结合试验、蚀斑减少中和试验以及对分段双链（ds）RNA基因组进行聚丙烯酰胺凝胶电泳（PAGE），对31株帕利亚姆血清群病毒进行了检测。虽然通过补体结合试验无法区分这些病毒，但通过蚀斑减少中和方法鉴定出了10种不同的病毒血清型。通过中和试验区分出的帕利亚姆群病毒具有独特的dsRNA图谱，而通过中和试验无法区分的病毒则具有相同的dsRNA图谱。在9年时间里，从澳大利亚的牛和库蠓中分离出了3种帕利亚姆血清型的20个毒株。当对这些分离株的基因组进行电泳检测时，给定血清型内病毒分离株的dsRNA图谱是相同的。
