Otegui Marisa S, Pennington Jannice G
Department of Botany, University of Wisconsin-Madison, 430 Lincoln Drive, Madison WI, USA.
Laboratory of Molecular and Cellular Biology, University of Wisconsin-Madison, 1525 Linden Drive, Madison WI, USA.
Microscopy (Oxf). 2019 Feb 1;68(1):69-79. doi: 10.1093/jmicro/dfy133.
Electron tomography (ET) approaches are based on the imaging of a biological specimen at different tilt angles by transmission electron microscopy (TEM). ET can be applied to both plastic-embedded and frozen samples. Technological advancements in TEM, direct electron detection, automated image collection, and imaging processing algorithms allow for 2-7-nm scale axial resolution in tomographic reconstructions of cells and organelles. In this review, we discussed the application of ET in plant cell biology and new opportunities for imaging plant cells by cryo-ET and other 3D electron microscopy approaches.
电子断层扫描（ET）方法基于通过透射电子显微镜（TEM）在不同倾斜角度对生物样本进行成像。ET可应用于塑料包埋和冷冻样本。TEM、直接电子检测、自动图像采集和图像处理算法方面的技术进步使得在细胞和细胞器的断层扫描重建中能够实现2 - 7纳米尺度的轴向分辨率。在本综述中，我们讨论了ET在植物细胞生物学中的应用以及通过冷冻ET和其他三维电子显微镜方法对植物细胞进行成像的新机遇。
