College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, PR China; Medical College, Guangxi University, Nanning, Guangxi, 530004, PR China.
Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Technology and Business University-BTBU, Beijing, 100048, PR China.
Chem Biol Interact. 2019 Jan 25;298:112-120. doi: 10.1016/j.cbi.2018.09.021. Epub 2018 Nov 16.
Oenothein B has a wide range of biological activities. The present study probed into the underlying mechanism on how Oenothein B inhibits the proliferation of a lung cancer line A549. Our results showed that Oenothein B effectively inhibited the proliferation of A549 cells by inducing apoptosis and arresting cells at G1 stage. Furthermore, Oenothein B not only increased the level of intracellular reactive oxygen species (ROS), but also induced the upregulation of intracellular apoptotic triggers (cleavage caspase-3, PARP, cytochrome c level in the cytosol, Bax). Moreover, ROS inhibitor (N-acetyl-L-cystein, NAC) and PI3K agonist (Insulin-like growth factor 1, IGF-1) could resist cell proliferation inhibition induced by Oenothein B, respectively. ROS inhibitor significantly abrogated the activation of caspase 3/7 and 9 in the presence of Oenothein B. Additionally, suppression of p-PI3K and p-Akt, p-NF-κB by Oenothein B could be compensated by treatment with ROS inhibitor. To summarize, these results demonstrated that Oenothein B was able to prevent cell proliferation probably via ROS-mediated PI3K/Akt/NF-κB signaling pathway.
橙皮苷 B 具有广泛的生物学活性。本研究探讨了橙皮苷 B 抑制肺癌 A549 细胞增殖的作用机制。结果表明，橙皮苷 B 通过诱导细胞凋亡和使细胞 G1 期阻滞来有效抑制 A549 细胞的增殖。此外，橙皮苷 B 不仅增加了细胞内活性氧（ROS）的水平，还诱导了细胞内凋亡触发因子（裂解 caspase-3、PARP、细胞质中的细胞色素 c 水平、Bax）的上调。此外，ROS 抑制剂（N-乙酰-L-半胱氨酸，NAC）和 PI3K 激动剂（胰岛素样生长因子 1，IGF-1）分别可以抵抗橙皮苷 B 诱导的细胞增殖抑制。ROS 抑制剂显著阻断了橙皮苷 B 存在时 caspase 3/7 和 9 的激活。此外，ROS 抑制剂可补偿橙皮苷 B 对 p-PI3K 和 p-Akt、p-NF-κB 的抑制作用。综上所述，这些结果表明，橙皮苷 B 可能通过 ROS 介导的 PI3K/Akt/NF-κB 信号通路来防止细胞增殖。
