Low and medium frequency auditory steady-state responses decrease during NREM sleep.

Previous research on auditory steady-state responses (ASSRs) demonstrated sensitivity of 40 Hz ASSR to changes in the level of arousal, both in sleep and in general anaesthesia. In this study we extended the range of stimulation frequencies, using also low and medium stimulation frequencies (4, 6, 8, 12, 20, 40 Hz) and studied their susceptibility to the loss of consciousness in NREM sleep (N2 and N3 stages). Effects of NREM sleep were examined in power domain with relative power (RP), and in phase domain using inter-trial phase coherence (PC) parameter. The activity in power domain was also compared to no-stimulation data. Regions displaying significant waking-NREM sleep differences were selected using non-parametric suprathreshold cluster test. For 4, 6, 20 and 40 Hz stimulation relative power of ASSRs was lower in NREM sleep, with maximal change for 40 Hz stimulation. This decrease was not seen in no-stimulation condition. For all stimulation frequencies (except 12 Hz) we observed decrease of phase coherence of ASSR during NREM sleep. Our results demonstrate that low and medium frequency ASSRs are state-sensitive, thus susceptible to loss of consciousness during NREM sleep. Diminishing of power and phase coherence may result from cortical down states and/or thalamic inhibition. Our results support possible use of low- and medium-frequency ASSRs for discrimination between states of altered consciousness and emphasize the role of the auditory system in determining these variations.