Embrapa Vegetables, Brasília, Brazil.
Department of Cell Biology, University of Brasilia, Brasília, Brazil.
Virus Res. 2019 Jan 15;260:49-52. doi: 10.1016/j.virusres.2018.11.008. Epub 2018 Nov 16.
A novel single-stranded RNA virus was detected in a whitefly (Bemisia tabaci) sample subjected to high-throughput sequencing. The 8293 nt-long genome presents a polyadenylated 3' end, and contains two ORFs encoding putative 1596 and 849 aa-long proteins. These putative proteins display significant similarity to replicase and capsid polyproteins, respectively, of discitroviruses. Its complete genome sequence shared the highest nucleotide identity (59.8%) with cricket paralysis virus (family Dicistroviridae, genus Cripavirus). Phylogenetic analyses showed that this new virus putative protein sequences clustered with those from members of Dicistroviridae. However, the replicase and capsid polyprotein sequences clustered with those of members of different genera, respectively to Aparavirus and Cripavirus. RT-PCR using newly collected adult and nymph whitefly samples confirmed the presence of this virus in field populations of B. tabaci. Genome sequence and organization, and polyproteins comparison indicate that this virus is a new species of the family Dicistroviridae. The name Bemisia-associated dicistrovirus 1 is proposed for this virus.
从高通量测序的烟粉虱（Bemisia tabaci）样本中检测到一种新型单链 RNA 病毒。该病毒基因组全长 8293nt，具有多聚腺苷酸化的 3'末端，包含两个 ORF，分别编码推测的 1596 和 849 个氨基酸长的蛋白质。这两种假定蛋白分别与双生病毒科的复制酶和衣壳多蛋白具有显著的相似性。其完整基因组序列与蟋蟀麻痹病毒（双生病毒科，Cripavirus 属）的核苷酸同源性最高（59.8%）。系统发育分析表明，该新病毒的假定蛋白序列与双生病毒科成员的序列聚类，而复制酶和衣壳多蛋白序列分别与 Aparavirus 和 Cripavirus 属成员的序列聚类。使用新收集的成年和若虫烟粉虱样本进行 RT-PCR 证实了该病毒在田间烟粉虱种群中的存在。基因组序列和组织以及多蛋白比较表明，该病毒是双生病毒科的一个新种。建议将这种病毒命名为烟粉虱-associated dicistrovirus 1。
