Laboratory of Insect Ecology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
Research Institute for Sustainable Humanosphere, Kyoto University, Kyoto, 611-0011, Japan.
Arch Virol. 2020 Nov;165(11):2715-2719. doi: 10.1007/s00705-020-04769-2. Epub 2020 Aug 10.
We report two novel RNA viruses from yellow crazy ants, (Anoplolepis gracilipes) detected using next-generation sequencing. The complete genome sequences of the two viruses were 10,662 and 8,238 nucleotides in length, respectively, with both possessing two open reading frames with three conserved protein domains. The genome organization is characteristic of members of the genus Triatovirus in the family Dicistroviridae. The two novel viruses were tentatively named "Anoplolepis gracilipes virus 1" and "Anoplolepis gracilipes virus 2" (AgrV-1 and AgrV-2). Phylogenetic analyses based on amino acid sequences of the non-structural polyprotein (ORF1) suggest that the two viruses are triatovirus-like viruses. This is the first report on the discovery of novel triatovirus-like viruses in yellow crazy ants with a description of their genome structure (two ORFs and conserved domains of RNA helicase, RNA-dependent RNA polymerase, and capsid protein), complete sequences, and viral prevalence across the Asia-Pacific region.
我们报告了两种从黄疯蚁（Anoplolepis gracilipes）中检测到的新型 RNA 病毒，使用下一代测序技术。这两种病毒的完整基因组序列分别为 10662 和 8238 个核苷酸长，均具有两个开放阅读框和三个保守蛋白结构域。基因组组织特征是双 RNA 病毒科双翅目病毒属的成员。这两种新型病毒被暂时命名为“黄疯蚁病毒 1”和“黄疯蚁病毒 2”（AgrV-1 和 AgrV-2）。基于非结构多蛋白（ORF1）的氨基酸序列进行的系统进化分析表明，这两种病毒是类似双翅目病毒的病毒。这是首次在黄疯蚁中发现新型类似双翅目病毒的报告，描述了它们的基因组结构（两个 ORF 和 RNA 解旋酶、RNA 依赖性 RNA 聚合酶和衣壳蛋白的保守结构域）、完整序列以及在亚太地区的病毒流行情况。
