Chen Yongheng, Tan Shan, Liu Mei, Li Jianming
a Department of Cardiovascular Internal Medicine , the First Affiliated Hospital of Changsha Medical University , Changsha City , P.R. China.
b Department of Anatomy , Changsha Medical University , Changsha City , P.R. China.
Scand Cardiovasc J. 2018 Dec;52(6):335-339. doi: 10.1080/14017431.2018.1546896. Epub 2019 Jan 25.
LncRNA TINCR has been reported to be involved in cardiac hypertrophy, while its involvement in diabetic cardiomyopathy is unknown.
In this study, myocardial biopsy and serum collected from patients with diabetic cardiomyopathy, diabetic patients without cardiopathy and healthy controls, and the expression of TINCR in those tissues was detected by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR). ROC curve analysis was performed to evaluate the diagnostic value of TINCR expression for diabetic cardiomyopathy. Human cardiomyocyte cells (Cat# 12440053, Thermo Fisher Scientific) were treated with high glucose, and the expression of TINCR was detected by qRT-PCR. TINCR expression was transfected into cardiomyocyte cells and cell apoptosis under high glucose treated was detected by cell apoptosis assay.
We found that TINCR expression level in myocardial biopsy and serum was significantly lower in patients with diabetic cardiomyopathy than in diabetic patients without cardiopathy and healthy controls, while no significant differences were found between diabetic patients without cardiopathy and healthy controls. TINCR expression level can be used to effective diagnose diabetic cardiomyopathy. High glucose treatment showed no significant effects on the expression of TINCR in human cardiomyocyte cells, and TINCR overexpression inhibited apoptosis of cardiomyocytes under high glucose treatment.
Therefore, we conclude that lncRNA TINCR is downregulated in diabetic cardiomyopathy and it can inhibit cardiomyocyte apoptosis.
据报道，长链非编码RNA TINCR参与心脏肥大，但其在糖尿病心肌病中的作用尚不清楚。
在本研究中，收集糖尿病性心肌病患者、无心脏病的糖尿病患者和健康对照者的心肌活检组织和血清，采用定量逆转录聚合酶链反应（qRT-PCR）检测这些组织中TINCR的表达。进行ROC曲线分析以评估TINCR表达对糖尿病心肌病的诊断价值。用人高糖处理人心肌细胞（货号12440053，赛默飞世尔科技公司），通过qRT-PCR检测TINCR的表达。将TINCR表达载体转染到心肌细胞中，通过细胞凋亡检测法检测高糖处理下的细胞凋亡情况。
我们发现，糖尿病性心肌病患者心肌活检组织和血清中TINCR的表达水平显著低于无心脏病的糖尿病患者和健康对照者，而无心脏病的糖尿病患者和健康对照者之间无显著差异。TINCR表达水平可用于有效诊断糖尿病心肌病。高糖处理对人心肌细胞中TINCR的表达无显著影响，而TINCR过表达可抑制高糖处理下心肌细胞的凋亡。
因此，我们得出结论，lncRNA TINCR在糖尿病心肌病中表达下调，并且它可以抑制心肌细胞凋亡。
