描述在加纳大阿克拉地区不同疟疾传播强度的社区中无症状的成年人和儿童体内的抗-EBA175RIII-V 情况。
Characterization of anti-EBA175RIII-V in asymptomatic adults and children living in communities in the Greater Accra Region of Ghana with varying malaria transmission intensities.
机构信息
Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana.
Present address: University Cheikh Anta DIOP, Dakar, Senegal.
出版信息
BMC Immunol. 2018 Nov 19;19(1):34. doi: 10.1186/s12865-018-0271-y.
BACKGROUND
Antibodies against Region III-V of the erythrocyte binding antigen (EBA) 175 (EBA175RIII-V) have been suggested to provide protection from malaria in a natural infection. However, the quality and quantity of naturally induced antibodies to EBA175RIII-V has not been fully characterized in different cohorts of Ghanaians. This study sought to determine the characteristics of antibodies against EBA175RIII-V in asymptomatic adults and children living in two communities of varying P. falciparum parasite prevalence in southern Ghana.
METHODS
Microscopic evaluation of thick and thin blood smears was used to identify asymptomatic Plasmodium falciparum carriage and indirect enzyme linked immunosorbent (ELISA) used to assess antibody concentrations and avidity.
RESULTS
Parasite carriage estimated by microscopy in Obom was 35.6% as opposed to 3.5% in Asutsuare. Levels of IgG, IgG1, IgG2, IgG3 and IgG4 against EBA175RIII-V in the participants from Obom were significantly higher (P < 0.05, Dunn's Multiple Comparison test) than those in Asutsuare. However the relative avidity of IgG antibodies against EBA175RIII-V was significantly higher (P < 0.0001, Mann Whitney test) in Asutsuare than in Obom.
CONCLUSIONS
People living in communities with limited exposure to P. falciparum parasites have low quantities of high avidity antibodies against EBA175RIII-V whilst people living in communities with high exposure to the parasites have high quantities of age-dependent but low avidity antibodies against EBA175RIII-V.
背景
针对红细胞结合抗原（EBA）175 区域 III-V（EBA175RIII-V）的抗体已被认为在自然感染中提供了对疟疾的保护。然而，在加纳不同人群中，针对 EBA175RIII-V 的自然诱导抗体的质量和数量尚未得到充分描述。本研究旨在确定在加纳南部两个疟原虫流行率不同的社区中，无症状成年人和儿童对 EBA175RIII-V 的抗体特征。
方法
使用厚和薄血涂片的显微镜评估来识别无症状的恶性疟原虫携带情况，并使用间接酶联免疫吸附（ELISA）来评估抗体浓度和亲和力。
结果
Obom 中通过显微镜估计的寄生虫携带率为 35.6%，而 Asutsuare 为 3.5%。来自 Obom 的参与者针对 EBA175RIII-V 的 IgG、IgG1、IgG2、IgG3 和 IgG4 水平明显更高（P < 0.05，Dunn's 多重比较检验），而 Asutsuare 的 IgG 抗体针对 EBA175RIII-V 的相对亲和力明显更高（P < 0.0001，Mann Whitney 检验）。
结论
生活在疟原虫暴露有限的社区中的人具有针对 EBA175RIII-V 的低数量高亲和力抗体，而生活在寄生虫暴露高的社区中的人具有高数量的年龄依赖性但低亲和力的针对 EBA175RIII-V 的抗体。
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