Sarafidis K, Efstathiou N, Begou O, Soubasi V, Agakidou E, Gika E, Theodoridis G, Drossou V
1 Department of Neonatology, School of Medicine Aristotle University of Thessaloniki, Thessaloniki, Greece.
School of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Hippokratia. 2017 Apr-Jun;21(2):80-84.
Metabolomics could provide valuable insights into hypoxemic-ischemic encephalopathy (HIE) revealing new disease-associated biochemical derangements. The study aimed to investigate urine metabolic changes in neonates with HIE compared to healthy controls, using targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS).
In this prospective, single-center study we enrolled neonates born at ≥ 36 weeks gestation with HIE (HIE group) and healthy controls (control group). We collected urine samples for metabolomic analysis on days one, three, and nine of life.
Twenty-one full-term newborns were studied, 13 in the HIE group and eight in the control group. Six of the affected neonates had moderate/severe HIE and seven mild HIE. Therapeutic hypothermia was applied only in four neonates with moderate/severe HIE. Multivariate and univariate statistical analysis showed a clear separation between the HIE and the control groups. Discriminant metabolites involved pyruvic acid, amino acids, acylcarnitines, inositol, kynurenine, hippuric acid, and vitamins.
We have identified a specific metabolic profile in neonates with HIE, adding to the existing knowledge on the disease biochemistry that may potentially help in biomarker development. HIPPOKRATIA 2017, 21(2): 80-84.
代谢组学可为缺氧缺血性脑病（HIE）提供有价值的见解，揭示新的疾病相关生化紊乱。本研究旨在使用靶向液相色谱 - 串联质谱法（LC-MS/MS），调查与健康对照相比，HIE新生儿的尿液代谢变化。
在这项前瞻性单中心研究中，我们纳入了孕龄≥36周的HIE新生儿（HIE组）和健康对照（对照组）。我们在出生后第1天、第3天和第9天收集尿液样本进行代谢组学分析。
共研究了21名足月儿，其中HIE组13名，对照组8名。6名受影响的新生儿患有中度/重度HIE，7名患有轻度HIE。仅4名中度/重度HIE新生儿接受了治疗性低温治疗。多变量和单变量统计分析显示HIE组和对照组之间有明显区分。判别性代谢物涉及丙酮酸、氨基酸、酰基肉碱、肌醇、犬尿氨酸、马尿酸和维生素。
我们已确定HIE新生儿的特定代谢谱，增加了对该疾病生物化学的现有认识，这可能有助于生物标志物的开发。《希波克拉底》2017年，21(2): 80 - 84。
