跑步机训练对肥胖小鼠氧化应激标志物和内源性抗氧化状态的影响

The Effect of Treadmill Treatment on Oxidative Stress Markers and Endogenous Antioxidant Status in Obesity Mice.

作者信息

Susantiningsih Tiwuk, Perdani Roro Rukmi Windi, Berawi Khairunnisa, Hadi Sutopo

机构信息

Departement of Physiology, Medical Faculty, Universitas Lampung, Jl. S. Brojonegoro No. 1, Gedong Meneng Bandar Lampung, 35145, Indonesia.

Departement of Chemistry, Faculty of Mathematics and Natural of Sciences, Universitas Lampung, Jl. S. Brojonegoro No. 1, Gedong Meneng Bandar Lampung, 35145, Indonesia.

出版信息

Open Access Maced J Med Sci. 2018 Oct 19;6(10):1803-1808. doi: 10.3889/oamjms.2018.397. eCollection 2018 Oct 25.

DOI:10.3889/oamjms.2018.397

PMID:30455752

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6236053/

Abstract

AIM

This study aimed to determine the effect of treadmill treatment on oxidative stress markers and endogenous antioxidant status seen from MDA, GSH, MnSOD enzyme specific activity and blood catalase of obese mice.

MATERIALS AND METHODS

This research is experimental laboratory research using post-test control design group only. The study lasted for 28 days and was divided into 4 groups of study, i.e., group K (normal control), KP (obesity control), P1 (obesity mice with 1 x 10-minute treadmill treatment a day), and P2 (obesity mice with 2 x 10-minute treadmill treatment a day).

RESULTS

The treadmill treatment had an effect on the improvement of the oxidative status of mice with a decrease of MDA level of obesity mice blood (p ≤ 0.05) compared to KP control. An elevated endogenous antioxidant status of obesity mice was seen from elevated GSH levels, MnSOD specific activity and blood catalase of obesity mice (p ≤ 0.05) compared with KP controls. Treatment of 1 x 10-minute treadmill per day decreased blood MDA level, increased GSH enzyme and increased specific activity of MnSOD enzyme and blood catalase of obese mice.

CONCLUSIONS

The 2 x 10-minute daily treadmill did not differ significantly in improving the oxidative status and endogenous antioxidant status compared with the treadmill 1 x 10 minutes a day (p ≥ 0.05).

摘要

目的

本研究旨在确定跑步机训练对肥胖小鼠氧化应激标志物及内源性抗氧化状态的影响，具体指标包括丙二醛（MDA）、谷胱甘肽（GSH）、锰超氧化物歧化酶（MnSOD）的酶活性以及血液过氧化氢酶。

材料与方法

本研究为实验性实验室研究，仅采用测试后对照组设计。研究持续28天，分为4组，即K组（正常对照组）、KP组（肥胖对照组）、P1组（每天进行1次10分钟跑步机训练的肥胖小鼠）和P2组（每天进行2次10分钟跑步机训练的肥胖小鼠）。

结果

与KP对照组相比，跑步机训练对改善小鼠氧化状态有影响，肥胖小鼠血液中MDA水平降低（p≤0.05）。与KP对照组相比，肥胖小鼠的内源性抗氧化状态升高，表现为肥胖小鼠的GSH水平、MnSOD酶活性及血液过氧化氢酶升高（p≤0.05）。每天进行1次10分钟的跑步机训练可降低肥胖小鼠血液中的MDA水平，增加GSH酶活性，提高MnSOD酶活性及血液过氧化氢酶水平。