Nkodo Joseph Marie Mendimi, Ateba Roger, Pambe Christiane Judith Ngo, Okono Angèle Clarisse Kabeyene, Oyono Jean Louis Essamé
Department of Morphological Sciences and Anatomy Pathological, Faculty of Medicine and Biomedical Sciences of the University of Yaounde 1, Cameroon.
Pan Afr Med J. 2018 Jun 22;30:158. doi: 10.11604/pamj.2018.30.158.14982. eCollection 2018.
Lymph node tuberculosis remains widespread in Cameroon. Our goal was to compare the diagnostic agreement between the hematein-eosin coloration technique and the Ziehl-Neelsen technique.
This study is a retrospective and comparative study realized in the Yaounde University Teaching Hospital over a period of 5 years. We needed to specify the diagnostic agreement for tuberculosis, first inter observer and secondly between the standard and special colorations. The data we collected allowed us to determine the agreement rates observed and the kappa (k) coefficients with linear weighting.
The 186 samples of the 1726 cases of tuberculosis of all locations represented a proportion of 10.78%. There were more male patients (65.05%) with a sex ratio (M:F) of 1.30. The average age was 24.21 ± 15.5 with the extremes from 5 to 68 years. The most represented age group was from 10 to 39 years. The two observers agreed in 93 cases using hematein eosin coloration (P = 83.87 %; k = 0.8109) and on 73 samples using the Ziehl-Neelsen coloration (P = 89.78 %; k = 0.7734). The two coloration techniques presented an agreement on 104 samples (P = 88.17 %; k = 0.8783).
The routine choice of the hematein eosin coloration technique not paired with the coloration technique of Ziehl-Neelsen can effectively alleviate the program for fighting tuberculosis in an environment of limited resources.
淋巴结结核在喀麦隆仍然普遍存在。我们的目标是比较苏木精 - 伊红染色技术与齐 - 尼氏染色技术之间的诊断一致性。
本研究是一项回顾性比较研究，在雅温得大学教学医院进行，为期5年。我们需要明确结核病的诊断一致性，首先是观察者之间的一致性，其次是标准染色和特殊染色之间的一致性。我们收集的数据使我们能够确定观察到的一致性率以及线性加权的kappa（k）系数。
在所有部位的1726例结核病例中，186份样本占比10.78%。男性患者更多（65.05%），性别比（男：女）为1.30。平均年龄为24.21±15.5岁，年龄范围为5至68岁。最具代表性的年龄组为10至39岁。两位观察者使用苏木精 - 伊红染色在93例病例中达成一致（P = 83.87%；k = 0.8109），使用齐 - 尼氏染色在73份样本中达成一致（P = 89.78%；k = 0.7734）。两种染色技术在104份样本上呈现一致性（P = 88.17%；k = 0.8783）。
在资源有限的环境中，常规选择不与齐 - 尼氏染色技术配对的苏木精 - 伊红染色技术可有效减轻结核病防治项目的负担。
