Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489, Berlin, Germany.
Bundesanstalt für Materialforschung und -prüfung, Richard-Willstätter-Str. 11, 12489, Berlin, Germany.
Anal Bioanal Chem. 2019 Jan;411(3):629-637. doi: 10.1007/s00216-018-1477-9. Epub 2018 Nov 20.
Drug biodistribution analyses can be considered a key issue in pharmaceutical discovery and development. Here, mass spectrometric imaging can be employed as a powerful tool to investigate distributions of drug compounds in biologically and medically relevant tissue sections. Both matrix-assisted laser desorption ionization-mass spectrometric imaging as molecular method and laser ablation inductively coupled plasma-mass spectrometric imaging as elemental detection method were applied to determine drug distributions in tissue thin sections. Several mouse organs including the heart, kidney, liver, and brain were analyzed with regard to distribution of Gadovist, a gadolinium-based contrast agent already approved for clinical investigation. This work demonstrated the successful detection and localization of Gadovist in several organs. Furthermore, the results gave evidence that gadolinium-based contrast agents in general can be well analyzed by mass spectrometric imaging methods. In conclusion, the combined application of molecular and elemental mass spectrometry could complement each other and thus confirm analytical results or provide additional information.
药物生物分布分析可以被视为药物发现和开发中的关键问题。在这里，质谱成像可以作为一种强大的工具，用于研究生物和医学相关组织切片中药物化合物的分布。基质辅助激光解吸电离-质谱成像作为分子方法和激光烧蚀电感耦合等离子体质谱成像作为元素检测方法都被应用于组织薄片中药物分布的测定。对包括心脏、肾脏、肝脏和大脑在内的几种小鼠器官进行了分析，以研究已批准用于临床研究的基于钆的造影剂 Gadovist 的分布情况。这项工作成功地检测和定位了几种器官中的 Gadovist。此外，结果表明，一般来说，基于钆的造影剂可以很好地通过质谱成像方法进行分析。总之，分子和元素质谱的联合应用可以相互补充，从而确认分析结果或提供额外的信息。
