Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Physiol Plant. 2019 Sep;167(1):48-63. doi: 10.1111/ppl.12865. Epub 2019 Jan 10.
Hypocotyl elongation is an early event in plant growth and development and is sensitive to fluctuations in light, temperature, water potential and nutrients. Most research on hypocotyl elongation has focused on the regulatory mechanism of a single environment factor. However, information about combined effects of multi-environment factors remains unavailable, and overlapping sites of the environmental factors signaling pathways in the regulation of hypocotyl elongation remain unclear. To identify how cross-talks among light intensity, temperature and water potential regulate hypocotyl elongation in Brassica rapa L. ssp. chinesis, a comprehensive isobaric tag for relative and absolute quantitation-based proteomic approach was adopted. In total, 7259 proteins were quantified, and 378 differentially expressed proteins (DEPs) were responsive to all three environmental factors. The DEPs were involved in a variety of biochemical processes, including signal transduction, cytoskeletal organization, carbohydrate metabolism, cell wall organization, protein modification and transport. The DEPs did not function in isolation, but acted in a large and complex interaction network to affect hypocotyl elongation. Among the DEPs, phyB was outstanding for its significant fold change in quantity and complex interaction networks with other proteins. In addition, changes of sensitivity to environmental factors in phyB-9 suggested a key role in the regulation of hypocotyl elongation. Overall, the data presented in this study show a profile of proteins interaction network in response to light intensity, temperature and water potential and provides molecular basis of hypocotyl elongation in B. rapa.
下胚轴伸长是植物生长发育的早期事件，对光、温度、水势和养分的波动敏感。大多数关于下胚轴伸长的研究都集中在单一环境因素的调节机制上。然而，关于多环境因素综合效应的信息仍然缺乏，并且在调节下胚轴伸长的环境因素信号通路中重叠的位点也不清楚。为了确定光强、温度和水势如何相互作用调节芸薹属植物的下胚轴伸长，采用了基于等压标签相对和绝对定量的蛋白质组学综合方法。共定量了 7259 种蛋白质，有 378 种差异表达蛋白（DEPs）对所有三种环境因素都有反应。这些 DEPs 参与了多种生化过程，包括信号转导、细胞骨架组织、碳水化合物代谢、细胞壁组织、蛋白质修饰和运输。这些 DEPs 不是孤立作用的，而是在一个庞大而复杂的相互作用网络中影响下胚轴伸长。在 DEPs 中，phyB 因其数量的显著变化和与其他蛋白质的复杂相互作用网络而引人注目。此外，phyB-9 对环境因素敏感性的变化表明其在调节下胚轴伸长中的关键作用。总的来说，本研究提供的蛋白质相互作用网络变化的数据显示了对光强、温度和水势的响应，并为芸薹属植物下胚轴伸长提供了分子基础。
