黎巴嫩的载脂蛋白E基因型：分布及其与高胆固醇血症和阿尔茨海默病的关联

APOE genotypes in Lebanon: distribution and association with hypercholesterolemia and Alzheimer's disease.

作者信息

El Shamieh Said, Costanian Christy, Kassir Rayan, Visvkis-Siest Sophie, Bissar-Tadmouri Nisrine

机构信息

Department of Medical Laboratory Sciences, Faculty of Health Sciences, Beirut Arab University, Beirut, Lebanon.

School of Kinesiology & Health Science, York University, Toronto, ON M3J1P3, Canada.

出版信息

Per Med. 2019 Jan;16(1):15-23. doi: 10.2217/pme-2018-0067. Epub 2018 Nov 20.

DOI:10.2217/pme-2018-0067

PMID:30457419

Abstract

AIM

We first investigated the distribution of APOE genotypes in Lebanese general population and Alzheimer's disease study (ADS) groups, and compared it with 1000 genomes populations. Then, we assessed eventual association between APOE genotypes, hypercholesterolemia and Alzheimer's disease (AD).

MATERIALS & METHODS: This cross-sectional study was conducted on 591 individuals. Clinical and biological data were collected, DNA was extracted and genotyped using Kompetitive allele specific PCR (KASP™) and PCR-restriction fragment length polymorphis (PCR-RFLP). Results: Prevalence of APOE genotypes in Lebanon was similar to that seen in Asian populations. APOE genotypes were not associated with hypercholesterolemia. A significant difference between APOE genotypes in AD cases versus controls and versus Lebanese general population was seen. Moreover, E4 allele was approximately threefold higher in Alzheimer's disease study patients when compared with the remaining individuals.

CONCLUSION

We established the distribution of APOE genotypes in the Lebanon and showed that in contrast to lipid profile, E4 correlates with AD.

摘要

目的

我们首先调查了黎巴嫩普通人群和阿尔茨海默病研究（ADS）组中载脂蛋白E（APOE）基因型的分布，并将其与千人基因组计划人群进行比较。然后，我们评估了APOE基因型、高胆固醇血症与阿尔茨海默病（AD）之间的潜在关联。

材料与方法

本横断面研究共纳入591名个体。收集临床和生物学数据，提取DNA，并使用竞争性等位基因特异性PCR（KASP™）和PCR-限制性片段长度多态性（PCR-RFLP）进行基因分型。结果：黎巴嫩APOE基因型的患病率与亚洲人群相似。APOE基因型与高胆固醇血症无关。AD病例与对照组以及与黎巴嫩普通人群的APOE基因型之间存在显著差异。此外，与其余个体相比，阿尔茨海默病研究患者中的E4等位基因大约高出三倍。