Dorsman J C, van Heeswijk W C, Grivell L A
Section for Molecular Biology, Department of Molecular Cell Biology, Amsterdam, The Netherlands.
Nucleic Acids Res. 1988 Aug 11;16(15):7287-301. doi: 10.1093/nar/16.15.7287.
Two abundant factors, GFI and GFII which interact with the 5' flanking regions of nuclear genes coding for proteins of the mitochondrial respiratory chain have been identified. In one case (subunit VIII of QH2: cytochrome c oxidoreductase) the binding sites for both factors overlap completely and their binding is mutually exclusive. For the other 5' regions tested the GFI and GFII binding sites do not coincide. Interestingly, binding sites for GFI and GFII are also present in or at the 3' ends of the coding regions of two genes of the PHO gene family and in DNA elements important for optimal ARS and CEN function respectively. The sites recognized by GFI conform to the consensus RTCRNNNNNNACGNR, while those recognized by GFII contain the element RTCACGTG. We speculate that GFI and GFII may play a role in different cellular processes, dependent on the context of their binding sites and that one of these processes may be the coordination of the expression of genes involved in mitochondrial biogenesis with the progress of the cell cycle.
已经鉴定出两种丰富的因子，即GFI和GFII，它们与编码线粒体呼吸链蛋白质的核基因的5'侧翼区域相互作用。在一种情况下（QH2：细胞色素c氧化还原酶的亚基VIII），两种因子的结合位点完全重叠，并且它们的结合是相互排斥的。对于测试的其他5'区域，GFI和GFII的结合位点不一致。有趣的是，GFI和GFII的结合位点也分别存在于PHO基因家族的两个基因的编码区域的3'末端或内部，以及对最佳ARS和CEN功能重要的DNA元件中。GFI识别的位点符合共有序列RTCRNNNNNNACGNR，而GFII识别的位点包含元件RTCACGTG。我们推测，GFI和GFII可能在不同的细胞过程中发挥作用，这取决于它们结合位点的背景，并且这些过程之一可能是线粒体生物发生相关基因的表达与细胞周期进程的协调。
