Dimitrijevic Marija V, Mitic Violeta D, Nikolic Jelena S, Djordjevic Aleksandra S, Mutic Jelena J, Stankov Jovanovic Vesna P, Stojanovic Gordana S
Faculty of Science and Mathematics, Department of Chemistry, University of Nis, Visegradska 33, 18000, Nis, Serbia.
Faculty of Chemistry, University of Belgrade, Studentski trg12-16, 11000, Belgrade, Serbia.
Chem Biodivers. 2019 Feb;16(2):e1800492. doi: 10.1002/cbdv.201800492. Epub 2019 Jan 30.
The goal of this research was a comprehensive analysis of four wild edible mushroom species, Cantharellus cinereus, Clavariadelphus pistillaris, Clitocybe nebularis and Hygrocybe punicea, which have not been analyzed so far. Extracts of different polarities have been prepared and evaluated for their antioxidant activities by DPPH, ABTS, FRAP, TRP and CUPRAC methods. For all extracts, total phenolic content was determined. Based on the analysis, it was shown that solvent type had a significant effect on the antioxidant capacities of mushroom extracts, so water extracts showed the highest activity. Furthermore, the analysis includes determination of mineral composition, fatty acid profiles and antimicrobial activity. Unsaturated fatty acids, which are very important for human health, are dominant in the studied mushroom species. Linoleic and oleic acid consist of over 50 % of the total fatty acid composition. Seventeen biologically important and toxic elements have been analyzed by ICP-OES and ICP-MS and results showed that the element concentrations were species-dependent. Also, it has been found that analyzed mushrooms did not show any antimicrobial activity. Chemometric analysis was used to understand the connection between the extracts of different polarities.
本研究的目标是对四种野生可食用蘑菇物种进行全面分析，这四种蘑菇分别是灰喇叭菌、柱状珊瑚菌、雾状杯伞和朱红蜡伞，目前尚未对它们进行过分析。制备了不同极性的提取物，并通过DPPH、ABTS、FRAP、TRP和CUPRAC方法评估其抗氧化活性。对所有提取物测定了总酚含量。基于分析结果表明，溶剂类型对蘑菇提取物的抗氧化能力有显著影响，因此水提取物显示出最高活性。此外，分析还包括矿物质组成、脂肪酸谱和抗菌活性的测定。对人体健康非常重要的不饱和脂肪酸在所研究的蘑菇物种中占主导地位。亚油酸和油酸占总脂肪酸组成的50%以上。通过电感耦合等离子体发射光谱法（ICP-OES）和电感耦合等离子体质谱法（ICP-MS）分析了17种具有生物学重要性和毒性的元素，结果表明元素浓度因物种而异。此外，还发现所分析的蘑菇没有显示出任何抗菌活性。采用化学计量学分析来了解不同极性提取物之间的联系。
