Department of Food Processing, Bahçe Vocational School, Osmaniye Korkut Ata University, Osmaniye, Turkey.
Department of Biology, Faculty of Science, Akdeniz University, Antalya, Turkey.
Oxid Med Cell Longev. 2020 Feb 19;2020:5620484. doi: 10.1155/2020/5620484. eCollection 2020.
(Bull.) Harmaja is an edible mushroom found in Bolu province in northwestern Turkey. The chemical composition and bioactivity of these mushrooms has not been previously investigated. We examined the phenolic composition, elemental content, and antioxidant and antigenotoxic effects of methanol extracts of fruiting bodies. The phenolic compounds in the fungal samples were determined using high-performance liquid chromatography (HPLC), and element content was determined using atomic absorption spectrophotometry. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were determined using the commercially available Rel assay kit. The antigenotoxic effects of the extract were determined using the MTT assay to assess cell viability and the alkaline single-cell gel electrophoresis assay (Comet assay). The total phenolic content (ppm) of was found to be catechin (361 ± 2.31), clorogenic acid (553.54 ± 5.06), and coumaric acid (9.93 ± 0.25). The TAS, TOS, and OSI of the extract were 1.854 ± 0.051 mmol/L, 30.385 ± 0.399 mol/L, and 1.639 ± 0.067, respectively. The elemental levels were within "normal" range. In HT22 mouse hippocampal neuronal cells, the extract (100 and 200 g/ml) showed no genotoxic potential and ameliorated hydrogen peroxide- (HO-) induced oxidative DNA damage. may be considered a good nutrient due to its phenolic constituents and antioxidant potential.
（Bull.）在土耳其西北部的博卢省发现了一种可食用的蘑菇，名为 Harmaja。这些蘑菇的化学成分和生物活性尚未被研究过。我们研究了子实体甲醇提取物的酚类成分、元素含量、抗氧化和抗原毒作用。使用高效液相色谱法（HPLC）测定真菌样品中的酚类化合物，原子吸收分光光度法测定元素含量。使用市售的 Rel 测定试剂盒测定总抗氧化状态（TAS）、总氧化状态（TOS）和氧化应激指数（OSI）。使用 MTT 测定法评估细胞活力和碱性单细胞凝胶电泳测定法（彗星测定法）来测定提取物的抗原毒作用。的总酚含量（ppm）被发现为儿茶素（361±2.31）、绿原酸（553.54±5.06）和香豆酸（9.93±0.25）。提取物的 TAS、TOS 和 OSI 分别为 1.854±0.051mmol/L、30.385±0.399mol/L 和 1.639±0.067。元素水平处于“正常”范围内。在 HT22 小鼠海马神经元细胞中，提取物（100 和 200μg/ml）没有表现出遗传毒性，并且改善了过氧化氢（HO-）诱导的氧化 DNA 损伤。由于其酚类成分和抗氧化潜力，可能被认为是一种良好的营养物质。
