大肠杆菌的色氨酸阻遏物表现出异常的热稳定性。
Tryptophan repressor of Escherichia coli shows unusual thermal stability.
作者信息
Bae S J, Chou W Y, Matthews K, Sturtevant J M
机构信息
Department of Chemistry, Yale University, New Haven, CT 06511.
出版信息
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6731-2. doi: 10.1073/pnas.85.18.6731.
Abstract
Differential scanning calorimetry demonstrates that the tryptophan repressor of Escherichia coli is unusually resistant to thermal denaturation. The dimeric protein undergoes reversible dissociative unfolding at pH 7.5 centered at about 90 degrees C. The thermal stability may be due in part to the unusual structure of the protein, which is composed of two identical intertwined polypeptide chains.
摘要
差示扫描量热法表明，大肠杆菌的色氨酸阻遏物对热变性具有异常的抗性。这种二聚体蛋白质在pH 7.5、约90℃时经历可逆的解离性解折叠。其热稳定性可能部分归因于该蛋白质的异常结构，它由两条相同的相互缠绕的多肽链组成。
相似文献
1
Tryptophan repressor of Escherichia coli shows unusual thermal stability.大肠杆菌的色氨酸阻遏物表现出异常的热稳定性。
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6731-2. doi: 10.1073/pnas.85.18.6731.
2
Differential scanning calorimetry of thermal unfolding of the methionine repressor protein (MetJ) from Escherichia coli.
Biochemistry. 1992 Oct 13;31(40):9717-24. doi: 10.1021/bi00155a027.
3
Thermodynamic and structural analysis of the folding/unfolding transitions of the Escherichia coli molecular chaperone DnaK.大肠杆菌分子伴侣DnaK折叠/去折叠转变的热力学和结构分析
J Mol Biol. 1993 Jul 20;232(2):680-92. doi: 10.1006/jmbi.1993.1418.
4
Thermal denaturation of the core protein of lac repressor.乳糖阻遏蛋白核心蛋白的热变性
Biochemistry. 1985 Jul 16;24(15):3842-6. doi: 10.1021/bi00336a004.
5
Stimulated interaction between and subunits of tryptophan synthase from hyperthermophile enhances its thermal stability.嗜热菌色氨酸合酶的α亚基和β亚基之间的刺激相互作用增强了其热稳定性。
J Biol Chem. 2003 Mar 14;278(11):8922-8. doi: 10.1074/jbc.m210893200.
6
Stability of the lac repressor headpiece against thermal denaturation and tryptic hydrolysis.乳糖阻遏蛋白头部结构域对热变性和胰蛋白酶水解的稳定性。
Biochim Biophys Acta. 1982 Apr 3;702(2):155-62. doi: 10.1016/0167-4838(82)90497-6.
7
Urea and thermal equilibrium denaturation studies on the dimerization domain of Escherichia coli Trp repressor.大肠杆菌色氨酸阻遏物二聚化结构域的尿素和热平衡变性研究。
Biochemistry. 1997 May 13;36(19):5612-23. doi: 10.1021/bi970056e.
8
Thermodynamic and kinetic stability of penicillin acylase from Escherichia coli.大肠杆菌青霉素酰化酶的热力学和动力学稳定性
Biochim Biophys Acta. 2008 May;1784(5):736-46. doi: 10.1016/j.bbapap.2008.01.016. Epub 2008 Feb 12.
9
Irreversible denaturation of maltodextrin glucosidase studied by differential scanning calorimetry, circular dichroism, and turbidity measurements.通过差示扫描量热法、圆二色性和浊度测量研究麦芽糖糊精葡萄糖苷酶的不可逆变性。
PLoS One. 2014 Dec 30;9(12):e115877. doi: 10.1371/journal.pone.0115877. eCollection 2014.
10
Scanning calorimetric study of the thermal unfolding of catabolite activator protein from Escherichia coli in the absence and presence of cyclic mononucleotides.在有无环单核苷酸存在的情况下，对来自大肠杆菌的分解代谢物激活蛋白热解折叠的扫描量热研究。
Biochemistry. 1988 Jul 12;27(14):5257-61. doi: 10.1021/bi00414a046.
引用本文的文献
1
The physical chemistry of high-sensitivity differential scanning calorimetry of biopolymers.生物聚合物的高灵敏度差示扫描量热法的物理化学
ChemTexts. 2017;3(1):1. doi: 10.1007/s40828-016-0038-0. Epub 2016 Dec 27.
2
Environment-dependent long-range structural distortion in a temperature-sensitive point mutant.温度敏感点突变体中依赖环境的长程结构扭曲。
Protein Sci. 2012 Jan;21(1):63-74. doi: 10.1002/pro.759. Epub 2011 Dec 8.
3
Mutant tryptophan aporepressors with altered specificities of corepressor recognition.具有改变的辅阻遏物识别特异性的突变型色氨酸aporepressors。
Genetics. 1991 May;128(1):29-35. doi: 10.1093/genetics/128.1.29.
本文引用的文献
1
Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor.大肠杆菌色氨酸脱辅基阻遏物的结构基因trpR的核苷酸序列及表达
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7117-21. doi: 10.1073/pnas.77.12.7117.
2
Functional inferences from crystals of Escherichia coli trp repressor.
J Biol Chem. 1983 Oct 25;258(20):12641-3.
3
The three-dimensional structure of trp repressor.色氨酸阻遏蛋白的三维结构。
Nature. 1985;317(6040):782-6. doi: 10.1038/317782a0.
4
Thermal denaturation of the core protein of lac repressor.乳糖阻遏蛋白核心蛋白的热变性
Biochemistry. 1985 Jul 16;24(15):3842-6. doi: 10.1021/bi00336a004.
5
High level production and rapid purification of the E. coli trp repressor.大肠杆菌色氨酸阻遏物的高水平生产与快速纯化
Nucleic Acids Res. 1986 Oct 24;14(20):7851-60. doi: 10.1093/nar/14.20.7851.
Zotero 插件