Department of Laboratory Medicine, Tumor Hospital of Ganzhou, Ganzhou, Jiangxi, China (mainland).
Department of Gynecology, Tumor Hospital of Ganzhou, Ganzhou, Jiangxi, China (mainland).
Med Sci Monit. 2018 Nov 20;24:8383-8390. doi: 10.12659/MSM.913346.
BACKGROUND The present study explored the expression of coiled-coil domain-containing 34 (CCDC34) in cervical cancer (CC) and its prognostic value. MATERIAL AND METHODS GEPIA and Oncomine cancer databases were mined to predict the CCDC34 differential expression level between a CC group and a normal group. Immunohistochemistry was performed to examine the CCDC34 expression in 67 CC and corresponding adjacent tissues. CD31 and vascular endothelial growth factor (VEGF) were stained to reflect tumor angiogenesis in 67 CC tissues. Kaplan-Meier univariate and Cox multivariate survival analysis were done to evaluate the correlation between CCDC34 expression and prognosis of CC patients. RESULTS Both GEPIA and Oncomine cancer databases mining results revealed that CCDC34 was more highly expressed in the CC group than in the normal group (all P<0.05). Our immunochemical staining data showed that CCDC34 expression was dramatically higher in CC than in adjacent normal tissues (71.6 vs. 20.9%; P<0.001). High expression of CCDC34 was strongly associated with histological grade (P=0.022), lymph node metastasis (P=0.044), and FIGO stage (P=0.002). Furthermore, patients with CCDC34-positive expression had much more MVD than those with CCDC34-negative expression (P<0.001). Kaplan-Meier survival analysis showed that CCDC34-positive expression was associated with worse overall survival (OS) (P=0.004) and disease-free survival (DFS) (P=0.005). Additionally, Cox multivariate analysis revealed that CCDC34 was an independent unfavorable prognostic parameter of DFS and OS (P=0.040 and 0.039, respectively). CONCLUSIONS High expression of CCDC34 is an independent unfavorable prognostic parameter for OS and DFS of CC patients, which was strongly associated with tumor angiogenesis.
本研究探讨卷曲螺旋结构域蛋白 34（CCDC34）在宫颈癌（CC）中的表达及其预后价值。
利用 GEPIA 和 Oncomine 癌症数据库预测 CC 组和正常组之间 CCDC34 的差异表达水平。采用免疫组织化学法检测 67 例 CC 及相应癌旁组织中 CCDC34 的表达。对 67 例 CC 组织中 CD31 和血管内皮生长因子（VEGF）进行染色，以反映肿瘤血管生成。采用 Kaplan-Meier 单因素和 Cox 多因素生存分析评估 CCDC34 表达与 CC 患者预后的相关性。
GEPIA 和 Oncomine 癌症数据库挖掘结果均显示，CC 组中 CCDC34 的表达高于正常组（均 P<0.05）。我们的免疫化学染色数据显示，CC 中 CCDC34 的表达明显高于癌旁正常组织（71.6%比 20.9%；P<0.001）。CCDC34 高表达与组织学分级（P=0.022）、淋巴结转移（P=0.044）和 FIGO 分期（P=0.002）密切相关。此外，CCDC34 阳性表达患者的 MVD 明显高于 CCDC34 阴性表达患者（P<0.001）。Kaplan-Meier 生存分析显示，CCDC34 阳性表达与总生存（OS）（P=0.004）和无病生存（DFS）（P=0.005）不良相关。此外，Cox 多因素分析显示，CCDC34 是 DFS 和 OS 的独立不良预后因素（P=0.040 和 0.039）。
CCDC34 高表达是 CC 患者 OS 和 DFS 的独立不良预后因素，与肿瘤血管生成密切相关。
