Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture & Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province , South China Agricultural University , Wushan Road 483 , Guangzhou , Guangdong 510642 , People's Republic of China.
Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute , Guangdong Academy of Agricultural Sciences , Guangzhou , Guangdong 510640 , People's Republic of China.
J Agric Food Chem. 2018 Dec 19;66(50):13107-13115. doi: 10.1021/acs.jafc.8b04900. Epub 2018 Dec 4.
Fusarium verticillioides is an important corn pathogen that can produce fumonisins (FBs) under certain environmental conditions. In this study, we evaluated the enantioselective impact of tebuconazole enantiomers on the growth and FB production of F. verticillioides on maize-based media at different abiotic factors. The expression of FB biosynthetic genes ( FUM1 and FUM6) was quantified by real-time reverse transcription polymerase chain reaction. The results showed that water activity ( a), temperature, and types of tebuconazole significantly affected the growth of F. verticillioides. The order of fungicidal activity was (-)-tebuconazole > rac-tebuconazole > (+)-tebuconazole. (-)-tebuconazole exhibited the maximal selective fungicidal activity (242-fold) against F. verticillioides at 0.95 a and 35 °C. Production of fumonisin B (FB) and fumonisin B (FB) by F. verticillioides was influenced by a, temperature, types of tebuconazole, and dose. Under most conditions, (-)-tebuconazole showed stronger inhibition for FB and FB production than (+)-tebuconazole (1.87-2.85-fold reduction in FBs) and rac-tebuconazole. The optimal environmental condition for FB production was at 0.99 a and 25 °C. Tebuconazole enantiomers differently affected FB biosynthetic gene ( FUM1 and FUM6) expression, but the effects on FB production and gene expression showed no positive correlation. The present study provides a better understanding on ways to minimize FB production in corn treated with fungicides.
镰刀菌是一种重要的玉米病原体，在某些环境条件下可以产生伏马菌素（FBs）。在这项研究中，我们评估了不同非生物因素下，丙硫菌唑对玉米基培养基中镰刀菌生长和 FB 产生的对映选择性影响。通过实时逆转录聚合酶链反应定量测定 FB 生物合成基因（FUM1 和 FUM6）的表达。结果表明，水活度（a）、温度和丙硫菌唑类型显著影响镰刀菌的生长。杀菌活性的顺序为（-）-丙硫菌唑>rac-丙硫菌唑>（+）-丙硫菌唑。（-）-丙硫菌唑在 0.95 a 和 35°C 时对镰刀菌表现出最大的选择性杀菌活性（242 倍）。伏马菌素 B（FB）和伏马菌素 B（FB）的产生受 a、温度、丙硫菌唑类型和剂量的影响。在大多数情况下，（-）-丙硫菌唑对 FB 和 FB 产生的抑制作用强于（+）-丙硫菌唑（FB 减少 1.87-2.85 倍）和 rac-丙硫菌唑。FB 产生的最佳环境条件为 0.99 a 和 25°C。丙硫菌唑对映异构体对 FB 生物合成基因（FUM1 和 FUM6）表达的影响不同，但对 FB 产生和基因表达的影响没有正相关。本研究为在使用杀菌剂处理的玉米中最大限度地减少 FB 产生提供了更好的理解。
