Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture& Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Tianhe District, Guangzhou 510642, China.
Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Agriculture& Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Wushan Road 483, Tianhe District, Guangzhou 510642, China.
Pestic Biochem Physiol. 2018 May;147:102-109. doi: 10.1016/j.pestbp.2017.12.010. Epub 2018 Jan 2.
Myclobutanil is a widely used triazole fungicide, comprising two enantiomers with different fungicidal activities, non-target toxicities, and environmental fates. The enantioselective effects of myclobutanil on fumonisin B (FB) production by Fusarium verticillioides, an important pathogen, have not yet been investigated. In the present study, the fungicidal activities of rac-myclobutanil and its enantiomers on F. verticillioides cultured on maize-based media were studied under different water activity and temperature conditions. The FB levels were measured to assess the enantioselective effects on FB production when F. verticillioides were cultured treated with EC and EC concentrations (concentrations inhibiting mycelial growth by 50.0% and 90.0%, respectively) of myclobutanil and enantiomers under different conditions. The fungicidal activities of rac-myclobutanil and its enantiomers decreased with increasing temperature and decreasing water activity. Little difference in fungicidal activity was observed between the enantiomers. FB production was significantly influenced by temperature, a, and fungicides dose. At EC50 concentrations, rac-myclobutantil and its enantiomers were shown to enhance mycotoxin production and enantioselective effects of enantiomers on FB production were observed under certain conditions. This is the first report on the differential effects of myclobutanil enantiomers on the control of F. verticillioides growth and FB production in maize-based media under different conditions.
环戊菌唑是一种广泛使用的三唑类杀菌剂，由两种具有不同杀菌活性、非靶标毒性和环境归宿的对映异构体组成。环戊菌唑对重要病原体串珠镰刀菌（Fusarium verticillioides）产生伏马菌素 B（FB）的对映选择性效应尚未得到研究。本研究在不同水活度和温度条件下，研究了 rac-环戊菌唑及其对映异构体对玉米基培养基上培养的 F. verticillioides 的杀菌活性。通过测量 FB 水平来评估当 F. verticillioides 在不同条件下用 EC 和 EC 浓度（分别抑制菌丝生长 50.0%和 90.0%的浓度）处理时，对 FB 产生的对映选择性效应。rac-环戊菌唑及其对映异构体的杀菌活性随温度升高和水活度降低而降低。对映异构体之间的杀菌活性差异很小。FB 产量受温度、a 和杀菌剂剂量的显著影响。在 EC50 浓度下，rac-环戊菌唑及其对映异构体表现出增强产毒作用，并且在某些条件下观察到对映异构体对 FB 产生的对映选择性效应。这是首次报道环戊菌唑对映异构体在不同条件下对玉米基培养基中 F. verticillioides 生长和 FB 产生的控制的差异影响。
