Asbakk K, Iversen O J
Department of Microbiology, University of Trondheim, Norway.
Scand J Immunol. 1988 Aug;28(2):195-202. doi: 10.1111/j.1365-3083.1988.tb02431.x.
Four murine monoclonal antibodies (MoAb) were raised against p27 isolated from psoriatic scale. The MoAb recognized the antigen on a subfraction (0.1-1%) of psoriatic lymphocytes as well as in skin biopsies from psoriatic patients. In contrast, the antigen could not be detected on lymphocytes or in skin biopsies from healthy controls. A sandwich ELISA assay using the MoAb for detection of p27 is described. This method, together with a competition antibody-binding assay for distinction of epitopes, demonstrates that the MoAb recognize four different antigenic determinants on p27.
针对从银屑病鳞屑中分离出的p27产生了四种鼠单克隆抗体（MoAb）。这些单克隆抗体识别银屑病淋巴细胞亚组分（0.1 - 1%）以及银屑病患者皮肤活检组织中的抗原。相比之下，在健康对照的淋巴细胞或皮肤活检组织中未检测到该抗原。描述了一种使用单克隆抗体检测p27的夹心酶联免疫吸附测定法。该方法与用于区分表位的竞争抗体结合测定法一起，证明单克隆抗体识别p27上的四种不同抗原决定簇。
