Clark S M, Lai E, Birren B W, Hood L
Division of Biology, California Institute of Technology, Pasadena 91125.
Science. 1988 Sep 2;241(4870):1203-5. doi: 10.1126/science.3045968.
A new instrument has been developed for the electrophoretic separation of large DNA molecules that can independently regulate the voltage of each of 24 electrodes and allow the magnitude, orientation, homogeneity, and duration of the electric field to be precisely controlled. Each parameter can be varied at any time during the electrophoretic process. Thus distinct sets of conditions can be combined to optimize the separation of various fragment sizes in a single run. Independent control of electrode voltage allows all of the fields to be generated with electrodes arranged in a closed contour, independent of a particular geometry. This device increases both the resolution in any size range and the speed of separation, especially for DNA molecules larger than 3 megabases.
一种用于大DNA分子电泳分离的新仪器已被开发出来，它可以独立调节24个电极中每个电极的电压，并能精确控制电场的大小、方向、均匀性和持续时间。在电泳过程中的任何时候，每个参数都可以变化。因此，可以将不同的条件组合起来，以便在一次运行中优化各种片段大小的分离。电极电压的独立控制使得所有电场都可以通过排列成闭合轮廓的电极产生，而与特定的几何形状无关。该装置提高了任何大小范围内的分辨率和分离速度，特别是对于大于3兆碱基的DNA分子。
