Cantor C R, Gaal A, Smith C L
Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032.
Biochemistry. 1988 Dec 27;27(26):9216-21. doi: 10.1021/bi00426a021.
The resolution of pulsed-field gel electrophoresis is dramatically affected by the number and configuration of the electrodes used, because these alter the shape of the applied electrical fields. Here we present calculations and experiments on the effect of electrode position in one of the most commonly used pulsed-field gel electrophoresis configurations. The goal was to explore which aspects of the electrical field shape correlate with improved electrophoretic resolution. The most critical variable appears to be the angle between the alternate electrical fields. The most effective electrode configurations yield angles of more than 110 degrees. A continually increasing angle between the fields produces band sharpening that greatly enhances the resolution.
脉冲场凝胶电泳的分辨率会受到所用电极数量和配置的显著影响，因为这些因素会改变外加电场的形状。在此，我们针对最常用的脉冲场凝胶电泳配置之一中电极位置的影响进行了计算和实验。目的是探究电场形状的哪些方面与电泳分辨率的提高相关。最关键的变量似乎是交替电场之间的夹角。最有效的电极配置会产生大于110度的夹角。电场之间夹角的持续增大可使条带锐化，从而极大地提高分辨率。
