Li Bo, Wu Dan, Cai Yingying, Vladimir G Monakhov, Zhang Wei, Xu Yanchun
College of Wildlife Resources, Northeast Forestry University, Harbin, People's Republic of China.
State Forestry Administration Detecting Center of Wildlife Resources, Harbin, People's Republic of China.
Anim Cells Syst (Seoul). 2018 Jul 9;22(4):253-258. doi: 10.1080/19768354.2018.1494039. eCollection 2018.
Genetic individualization based on non-invasive sampling is crucial for estimating the numbers of individuals in endangered mammalian populations. In sable ()-poaching cases, identifying the number of animals involved is critical for determining the penalty. In addition, investigating animal numbers for wild sable populations requires genetic individualization when collecting several samples in neighboring regions. Microsatellites have been demonstrated to be reliable markers for individual identification. Thirty-three microsatellite loci derived from Mustelidae were selected to develop a genetic individualization method for sable. Three reference populations containing 54 unrelated sables were used to calculate allele number, allelic frequencies, and the polymorphic information content of each locus. The data were subsequently used to assess the validity of a combination of twelve loci for sable individualization. We defined twelve polymorphic loci that were easy to be amplified and genotyped. Four significant deviations from Hardy-Weinberg equilibrium were observed among the 12 loci in the three populations. The match probability of an individual from the reference populations with a random individual based on the 12 loci was 1.37 × 10. Using the combination of the twelve loci provides sufficient power to individualize sables considering the levels of microsatellite polymorphism observed. These loci were successfully applied to a case of sable poaching and provided valid evidence to determine the penalty. The genetic individualization of sable based on these loci might also be useful to investigate the numbers of animals in wild populations.
基于非侵入性采样的遗传个体化对于估算濒危哺乳动物种群中的个体数量至关重要。在紫貂（）偷猎案件中，确定涉案动物的数量对于量刑至关重要。此外，在相邻地区采集多个样本时，对野生紫貂种群的动物数量进行调查需要进行遗传个体化。微卫星已被证明是用于个体识别的可靠标记。从鼬科动物中选取了33个微卫星位点，以开发一种紫貂的遗传个体化方法。使用包含54只无亲缘关系紫貂的三个参考种群来计算每个位点的等位基因数量、等位基因频率和多态信息含量。随后，这些数据被用于评估12个位点组合用于紫貂个体化的有效性。我们定义了12个易于扩增和基因分型的多态性位点。在这三个种群的12个位点中观察到四个显著偏离哈迪-温伯格平衡的情况。基于这12个位点，参考种群中一个个体与随机个体的匹配概率为1.37×10。考虑到观察到的微卫星多态性水平，使用这12个位点的组合为紫貂个体化提供了足够的能力。这些位点已成功应用于一起紫貂偷猎案件，并为量刑提供了有效证据。基于这些位点的紫貂遗传个体化对于调查野生种群中的动物数量可能也有用。
