辅酶Q和硒对部分单侧输尿管梗阻大鼠肾脏的影响。
The effect of coenzyme Q and selenium on kidney in rats with partial unilateral ureteral obstruction.
作者信息
Kırdağ Mustafa Koray, Tuğlu Devrim, Yuvanç Ercan, Kısa Üçler, Balcı Mahi, Batislam Ertan, Yılmaz Erdal
机构信息
Department of Urology, Kırıkkale University School of Medicine, Kırıkkale, Turkey.
Department of Biochemistry, Kırıkkale University School of Medicine, Kırıkkale, Turkey.
出版信息
Turk J Urol. 2019 Dec;45(Supp. 1):S70-S77. doi: 10.5152/tud.2018.22556. Epub 2018 Nov 19.
OBJECTIVE
In this study, we aimed to investigate the antioxidant effects of selenium and coenzyme Q on renal damage in a partial unilateral ureteral obstruction (PUUO) in a rat model.
MATERIAL AND METHODS
A total of 24 Sprague-Dawley rats were divided into four groups as Group 1 Control Group, Group 2, PUUO Group, Group 3 PUUO + coenzyme Q group, Group 4 PUUO + selenium group. Paraoxonase (PON), total antioxidant capacity (TAC), and total oxidant levels (TOS) were analyzed biochemically from tissue and blood samples. Tissue samples were examined histopathologically.
RESULTS
The TAC in the tissues was found to be statistically significantly increased in Groups 3 and 4, compared to Group 2. Tissue TOS was found to be significantly reduced in Groups 3 and 4, compared to Group 2. Serum PON levels were significantly increased in Group 3 and 4, compared to Group 1 and 2. Histopathological examination showed that interstitial inflammation and congestion were lesser in the coenzyme Q and selenium groups than in the PUUO group. A more significant decrease was found in the selenium group than in the coenzyme Q group.
CONCLUSION
Our study results showed that coenzyme Q and selenium reduced the oxidation and the damage in tissue in PUUO in rats.
目的
在本研究中，我们旨在探讨硒和辅酶Q对大鼠单侧输尿管部分梗阻（PUUO）所致肾损伤的抗氧化作用。
材料与方法
将24只Sprague-Dawley大鼠分为四组，即第1组对照组、第2组PUUO组、第3组PUUO + 辅酶Q组、第4组PUUO + 硒组。从组织和血液样本中对对氧磷酶（PON）、总抗氧化能力（TAC）和总氧化剂水平（TOS）进行生化分析。对组织样本进行组织病理学检查。
结果
与第2组相比，第3组和第4组组织中的TAC在统计学上显著升高。与第2组相比，第3组和第4组的组织TOS显著降低。与第1组和第2组相比，第3组和第4组的血清PON水平显著升高。组织病理学检查显示，辅酶Q组和硒组的间质炎症和充血程度低于PUUO组。硒组的降低程度比辅酶Q组更显著。
结论
我们的研究结果表明，辅酶Q和硒可减轻大鼠PUUO中组织的氧化和损伤。
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