TOM 受体招募细胞质 J 蛋白促进线粒体蛋白生物发生。
Recruitment of Cytosolic J-Proteins by TOM Receptors Promotes Mitochondrial Protein Biogenesis.
机构信息
Institute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany.
Institute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.
出版信息
Cell Rep. 2018 Nov 20;25(8):2036-2043.e5. doi: 10.1016/j.celrep.2018.10.083.
Mitochondria possess elaborate machineries for the import of proteins from the cytosol. Cytosolic factors like Hsp70 chaperones and their co-chaperones, the J-proteins, guide proteins to the mitochondrial surface. The translocase of the mitochondrial outer membrane (TOM) forms the entry gate for preproteins. How the proteins are delivered to mitochondrial preprotein receptors is poorly understood. We identify the cytosolic J-protein Xdj1 as a specific interaction partner of the central receptor Tom22. Tom22 recruits Xdj1 to the mitochondrial surface to promote import of preproteins and assembly of the TOM complex. Additionally, we find that the receptor Tom70 binds a different cytosolic J-protein, Djp1. Our findings suggest that cytosolic J-proteins target distinct TOM receptors and promote the biogenesis of mitochondrial proteins.
线粒体拥有精细的机制来从细胞质中导入蛋白质。细胞质中的因子，如 Hsp70 伴侣和它们的共伴侣 J 蛋白，引导蛋白质到达线粒体表面。线粒体外膜转位酶（TOM）形成前体蛋白的入口门。然而，蛋白质如何被递送到线粒体前体蛋白受体尚不清楚。我们鉴定了细胞质 J 蛋白 Xdj1 作为中央受体 Tom22 的特定相互作用伙伴。Tom22 将 Xdj1 招募到线粒体表面，以促进前体蛋白的导入和 TOM 复合物的组装。此外，我们发现受体 Tom70 结合了不同的细胞质 J 蛋白 Djp1。我们的发现表明，细胞质 J 蛋白靶向不同的 TOM 受体，并促进线粒体蛋白的生物发生。
Zotero 插件