Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, Japan.
Department of Advanced Biomaterials, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo, Japan.
J Bone Miner Metab. 2019 Jul;37(4):676-684. doi: 10.1007/s00774-018-0975-3. Epub 2018 Nov 22.
Medication-related osteonecrosis of the jaw (MRONJ) is a serious adverse effect of antiresorptive agents like bisphosphonates. Abnormal concentrations of various trace metallic elements contained in bone minerals have been associated with MRONJ. In this study, we focused on trace metallic elements contained in the MRONJ sequestrum; their content and distribution were compared to those in osteomyelitis and non-inflammatory bones using inductively coupled plasma atomic emission spectroscopy (ICP-AES) and synchrotron radiation X-ray fluorescence analysis (SR-XRF). On ICP-AES analyses, various trace elements (Co, Cr, Cu, Fe, K, Mg, Ni, Sb, Ti, V, Pb) were significantly more in MRONJ sequestra than non-inflammatory bones. The Cu content was significantly higher in MRONJ sequestra than osteomyelitis and non-inflammatory bones. The Cu content in MRONJ sequestra was high even after decalcification. Additionally, Cu was distributed along the trabecular structures in decalcified MRONJ specimens, as observed using SR-XRF analysis. Therefore, this study was indicative of the characteristic behavior of Cu in MRONJ.
药物相关性颌骨坏死（MRONJ）是双膦酸盐等抗吸收剂的严重不良反应。骨矿物质中所含的各种痕量金属元素的异常浓度与 MRONJ 有关。在这项研究中，我们专注于 MRONJ 死骨中所含的痕量金属元素；使用电感耦合等离子体原子发射光谱法（ICP-AES）和同步辐射 X 射线荧光分析（SR-XRF），比较了它们在骨髓炎和非炎症骨中的含量和分布。在 ICP-AES 分析中，MRONJ 死骨中的各种痕量元素（Co、Cr、Cu、Fe、K、Mg、Ni、Sb、Ti、V、Pb）明显多于非炎症骨。MRONJ 死骨中的 Cu 含量明显高于骨髓炎和非炎症骨。即使在脱钙后，MRONJ 死骨中的 Cu 含量仍然很高。此外，使用 SR-XRF 分析观察到，Cu 沿着脱钙的 MRONJ 标本中的小梁结构分布。因此，这项研究表明 Cu 在 MRONJ 中的特征行为。
