Sugiyama Tomoko, Uo Motohiro, Mizoguchi Teruyasu, Wada Takahiro, Omagari Daisuke, Komiyama Kazuo, Mori Yoshiyuki
Department of Dentistry, Oral and Maxillofacial Surgery, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan.
Advanced Biomaterials Department, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan; Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Bone Rep. 2015 Aug 10;3:40-47. doi: 10.1016/j.bonr.2015.08.001. eCollection 2015 Dec.
Bisphosphonates (BPs) have been widely, efficiently, and safely used for the treatment of various bone-related diseases such as osteoporosis. However, concerns about jaw osteonecrosis associated with oral treatment (medication-related osteonecrosis of the jaw [MRONJ]) have been increasing. Although many risk factors for MRONJ have been elucidated, its precise etiology and methods of prevention remain unknown. In this study, we have applied various elemental analysis methods for MRONJ specimens (e.g., X-ray fluorescence with synchrotron radiation [SR-XRF], particle-induced X-ray emission [PIXE], X-ray absorption fine structure [XAFS]) in order to reveal the accumulation and chemical state of trace bone minerals. In four MRONJ sequestra, the characteristic localization of copper (Cu) was observed by SR-XRF. Using micro-PIXE analysis, Cu looked to be localized near the edge of the trabecular bone. The chemical state of the accumulated Cu was estimated using XAFS and the possibility of a Cu-BP complex formation was assumed. Thus, in this study we argue for the feasibility of the trace element analysis to evaluate the potential pathophysiological mechanism of MRONJ.
双膦酸盐（BPs）已被广泛、有效且安全地用于治疗各种与骨相关的疾病，如骨质疏松症。然而，与口腔治疗相关的颌骨坏死（药物相关性颌骨坏死[MRONJ]）的担忧日益增加。尽管已经阐明了许多MRONJ的危险因素，但其确切病因和预防方法仍然未知。在本研究中，我们对MRONJ标本应用了各种元素分析方法（例如，同步辐射X射线荧光[SR-XRF]、粒子诱导X射线发射[PIXE]、X射线吸收精细结构[XAFS]），以揭示微量骨矿物质的积累和化学状态。在四个MRONJ死骨片中，通过SR-XRF观察到了铜（Cu）的特征性定位。使用微PIXE分析，Cu似乎定位于小梁骨边缘附近。使用XAFS估计了积累的Cu的化学状态，并假设了Cu-BP复合物形成的可能性。因此，在本研究中，我们论证了微量元素分析评估MRONJ潜在病理生理机制的可行性。
