Boujida Nadia, Palau Montserrat, Charfi Saoulajan, Manresa Àngels, Skali Senhaji Nadia, Abrini Jamal, Miñana-Galbis David
1Biotechnology and Applied Microbiology Research Group, Department of Biology, Faculty of Sciences, University Abdelmalek Essaâdi, BP2121, 93002 Tetouan, Morocco.
2Secció de Microbiologia, Department of Biologia, Sanitat i Medi Ambient, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Av. Joan XXIII, 27-31, 08028 Barcelona, Catalonia, Spain.
Int J Syst Evol Microbiol. 2019 Jan;69(1):227-234. doi: 10.1099/ijsem.0.003134. Epub 2018 Nov 22.
During the taxonomic investigation of exopolymer-producing halophilic bacteria, a rod-shaped, motile, Gram-stain-negative, halophilic bacterium, designated strain N4, was isolated from a saline soil located in northern Morocco. Optimal growth of the isolate was at 30-37 ºC and at pH 7.0-8.0, in the presence of 5-7 % (w/v) NaCl. Useful characteristics for the phenotypic differentiation of strain N4 from other Marinobacter species included α-chymotrypsin and α-glucosidase activities and the carbohydrate assimilation profile. The major fatty acids detected in strain N4 were C16:0 and C18:1ω9c and the predominant respiratory quinone was ubiquinone-9. Sequence analysis of the 16S rRNA gene indicated that strain N4 belonged to the genus Marinobacter and was closely related to the type strains of Marinobacter adhaerens (99.04 % similarity), Marinobacter salsuginis (98.97 %) and Marinobacter flavimaris (98.36 %). Phylogenetic analysis of the rpoD gene sequence also showed that the nearest neighbours of strain N4 were M. salsuginis (91.49 % similarity), M. adhaerens and M. flavimaris (90.63 %). Strain N4 showed 87.98 % average nucleotide identity with M. flavimaris and M. salsuginis, and 87.47 % with M. adhaerens. Regarding in-silico genome-to-genome distance, strain N4 showed DNA-DNA hybridization values of 33.30 % with M. adhaerens, 34.60 % with M. flavimaris and 34.70 % with M. salsuginis. The DNA G+C content of strain N4 was 57.3 mol%. Based on the results of phenotypic characterization, phylogenetic analysis and genome comparison, strain N4 represents a novel species of the genus Marinobacter, for which the name Marinobacter maroccanus sp. nov. is proposed. The type strain is N4 (=CECT 9525=LMG 30466).
在对产胞外聚合物嗜盐细菌进行分类学研究期间，从摩洛哥北部的盐渍土壤中分离出一株杆状、具运动性、革兰氏染色阴性的嗜盐细菌，命名为菌株N4。该分离株的最适生长温度为30 - 37 ºC，最适pH值为7.0 - 8.0，在5 - 7 %（w/v）NaCl存在的条件下生长。菌株N4与其他海杆菌属物种在表型上进行区分的有用特征包括α - 胰凝乳蛋白酶和α - 葡萄糖苷酶活性以及碳水化合物同化谱。在菌株N4中检测到的主要脂肪酸为C16:0和C18:1ω9c，主要呼吸醌为泛醌 - 9。16S rRNA基因序列分析表明，菌株N4属于海杆菌属，与海粘连海杆菌（相似度99.04 %）、盐渍海杆菌（98.97 %）和黄色海杆菌（98.36 %）的模式菌株密切相关。rpoD基因序列的系统发育分析也表明，菌株N4的最接近亲缘种是盐渍海杆菌（相似度91.49 %）、海粘连海杆菌和黄色海杆菌（90.63 %）。菌株N4与黄色海杆菌和盐渍海杆菌的平均核苷酸同一性为87.98 %，与海粘连海杆菌的平均核苷酸同一性为87.47 %。关于计算机模拟的全基因组到全基因组距离，菌株N4与海粘连海杆菌的DNA - DNA杂交值为33.30 %，与黄色海杆菌的为34.60 %，与盐渍海杆菌的为34.70 %。菌株N4的DNA G + C含量为57.3 mol%。基于表型特征、系统发育分析和基因组比较的结果，菌株N4代表海杆菌属的一个新物种，为此提出名称为摩洛哥海杆菌（Marinobacter maroccanus sp. nov.）。模式菌株为N4（=CECT 9525 = LMG 30466）。
