School of Environmental Science and Engineering, Shanghai University, Shanghai, China.
Lab for Noncoding RNA & Cancer, School of Life Sciences, Shanghai University, Shanghai, China.
Diabetes Metab Res Rev. 2019 Feb;35(2):e3104. doi: 10.1002/dmrr.3104. Epub 2018 Dec 19.
Diabetic wounds are refractory and very difficult to heal. We aimed to use miRNA to identify novel and specific molecular markers for diabetes mellitus (DM) diagnosis and treatment.
The expression level of miR-296-5p was determined in tissue samples of 12 DM patients. The effect of miR-296-5p on proliferation of β-cells was examined using Cell Counting Kit-8 (CCK-8) and colony formation assay. The effect of miR-296-5p on cell cycle progression was analysed using flow cytometry. The target gene was verified using luciferase reporter assay. A rat diabetes model was used to assess the effect of miR-296-5p in vivo.
Overexpression of miR-296-5p suppressed cell proliferation, arrested cell cycle progression, and increased the healing rate of diabetic wounds both in vivo and in vitro. TargetScan analysis results showed that miR-296-5p is a direct regulator of SGLT2.
miR-296-5p can increase the healing rate of diabetic wounds and may be an effective molecular tool in DM diagnosis and therapy.
糖尿病伤口难以愈合且具有抗药性。我们旨在利用 miRNA 来鉴定糖尿病（DM）诊断和治疗的新型特异性分子标志物。
检测了 12 例 DM 患者组织样本中的 miR-296-5p 表达水平。使用细胞计数试剂盒（CCK-8）和集落形成实验检测 miR-296-5p 对 β 细胞增殖的影响。通过流式细胞术分析细胞周期进程的影响。使用荧光素酶报告基因检测验证靶基因。利用大鼠糖尿病模型评估 miR-296-5p 在体内的作用。
miR-296-5p 的过表达抑制细胞增殖，使细胞周期停滞，并在体内和体外均增加糖尿病伤口的愈合率。TargetScan 分析结果表明，miR-296-5p 是 SGLT2 的直接调节因子。
miR-296-5p 可提高糖尿病伤口的愈合率，可能成为 DM 诊断和治疗的有效分子工具。
