Szacawa E, Jawor P, Dudek K, Bednarek D, Stefaniak T
Department of Cattle and Sheep Diseases, National Veterinary Research Institute, Partyzantow 57 Avenue, 24-100 Pulawy, Poland.
Department of Immunology, Pathophysiology and Veterinary Preventive Medicine, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 31 str., 50-375 Wroclaw, Poland.
Pol J Vet Sci. 2018 Sep;21(3):441-444. doi: 10.24425/122616.
Perinatal calf mortality in dairy herds has been reported worldwide. The etiology of stillbirth is multifactorial, and can be caused by various species of bacteria and environmental factors. Among them some potential pathogens from the Mollicutes class such as Mycoplasma (M.) spp. and Ureaplasma (U.) diversum can be isolated from the bovine genital tract and other organs of the suspected cattle. The aim of this study was to evaluate if the bacteria belonging to the Molli- cutes class i.e. M. bovis, M. bovigenitalium, M. canadense, M. canis, M. arginini, M. bovirhinis, M. dispar, M. alkalescens and U. diversum could have an impact on perinatal calf mortality in selected Polish dairy farms. The material was: 121 stillborn calves (SB), 21 live born calves (C) and 131 cows (dams) from 30 Polish Holstein-Friesian herds. Samples were examined from all the SB calves' and six control euthanized calves' abomasal contents and lung samples collected during necropsy, and from the dams' serum and placenta. In dams the serological ELISA, and in calves and placenta samples molecular PCR/denaturing gradient gel electrophoresis, methods were used. Screening of dams' sera for antibodies to M. bovis (ELISA) showed seven dams positive for M. bovis, whereas none of the nine examined Mollicutes microorganisms were detected in the placenta and calves.
围产期犊牛死亡率在全球范围内的奶牛场中均有报道。死产的病因是多因素的，可由多种细菌和环境因素引起。其中，一些来自柔膜菌纲的潜在病原体，如支原体属（M.）和脲原体属（U.）中的某些种类，可从疑似患病牛的生殖道和其他器官中分离出来。本研究的目的是评估属于柔膜菌纲的细菌，即牛支原体（M. bovis）、牛生殖支原体（M. bovigenitalium）、加拿大支原体（M. canadense）、犬支原体（M. canis）、精氨酸支原体（M. arginini）、牛鼻支原体（M. bovirhinis）、差异支原体（M. dispar）、碱性支原体（M. alkalescens）和多样脲原体（U. diversum）是否会对波兰选定奶牛场的围产期犊牛死亡率产生影响。材料包括：来自30个波兰荷斯坦-弗里生牛群的121头死产犊牛（SB）、21头活产犊牛（C）和131头母牛（母畜）。在尸检期间从所有死产犊牛以及6头对照安乐死犊牛的皱胃内容物和肺样本中取样，还从母畜的血清和胎盘中取样。对母畜采用血清学ELISA方法，对犊牛和胎盘样本采用分子PCR/变性梯度凝胶电泳方法。对母畜血清进行牛支原体抗体筛查（ELISA）显示7头母畜牛支原体呈阳性，而在所检测的胎盘中及犊牛中均未检测到9种柔膜菌微生物中的任何一种。
