Institute of Green Chemistry and Chemical Technology, School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
School of the Environment and Safety Engineering, Jiangsu University, Jiangsu, 212013, China.
Small. 2019 Jan;15(1):e1803913. doi: 10.1002/smll.201803913. Epub 2018 Nov 23.
Paper-based assays for detection of physiologically important species are needed in medical theranostics owning to their superiorities in point of care testing, daily monitoring, and even visual readout by using chromogenic materials. In this work, a facile test strip is developed for visual detection of a neurotransmitter dopamine (DA) based on dual-emission fluorescent molecularly imprinted polymer nanoparticles (DE-MIPs). The DE-MIPs, featured with tailor-made DA affinity and good anti-interference, exhibit DA concentration-dependent fluorescent colors, due to the variable ratios of dual-emission fluorescence caused by DA binding and quenching. By facile coating DE-MIPs on a filter paper, the DA test strips are obtained. The resultant test strip, like the simplicity of a pH test paper, shows the potential for directly visual detection of DA levels just by dripping a tiny amount of biofluid sample on it. The test result of real serum samples demonstrates that the DA strip enables to visually and semiquantitatively detect DA within 3 min by using only 10 µL of serum samples and with a low detection limit ((100-150) × 10 m) by naked eye. This work thus offers a facile and efficient strategy for rapid, visual, and on-site detection of biofluids in clinic.
基于显色材料的在医护现场即时检测、日常监测甚至可视化读取等优势，用于检测生理相关物质的纸基分析器件在医学诊断和治疗领域受到了广泛关注。在本工作中，我们开发了一种基于双发射荧光分子印迹聚合物纳米粒子（DE-MIPs）的简易测试条，用于可视化检测神经递质多巴胺（DA）。DE-MIPs 具有定制的 DA 亲和力和良好的抗干扰能力，由于 DA 结合和猝灭引起的双发射荧光比率变化，表现出与 DA 浓度相关的荧光颜色。通过简单地将 DE-MIPs 涂覆在滤纸上，即可获得 DA 测试条。与 pH 测试纸一样简单，得到的测试条只需将少量生物流体样本滴在上面，就可以直接可视化检测 DA 水平，具有潜在的应用前景。对实际血清样本的测试结果表明，该 DA 条可在 3 分钟内通过仅使用 10 µL 的血清样本进行可视化和半定量检测，且肉眼检测限低至（100-150）×10 m。因此，这项工作为临床中生物流体的快速、可视化和现场检测提供了一种简便、高效的策略。
