Kapoor Ashish, Raghunathan Muthukumar, Kumar Praveen, Tripathi S C, Haque Shafiul, Pal Dan Bahadur
Department of Chemical Engineering, Harcourt Butler Technical University, Kanpur, Uttar Pradesh India.
Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Chennai, Tamil Nadu 603203 India.
Indian J Microbiol. 2025 Mar;65(1):69-91. doi: 10.1007/s12088-024-01300-y. Epub 2024 May 18.
Molecularly imprinted polymers (MIPs) function as versatile and highly selective elements in biosensing, mimicking biomolecular receptors and effectively interacting with target analytes within complex matrices. Integrating MIPs with paper-based analytical devices (PADs) allows for rapid, convenient, and cost-effective deployment of molecular imprinting technologies. This review provides an overview of the advances in the fabrication process of MIP-PADs and explores their diverse applications, highlighting their utility in on-site detection using various detection mechanisms such as colorimetric, fluorometric, chemiluminescent electrochemical, photoelectrochemical, and surface enhanced Raman spectroscopy. The fabrication process involves synthesizing MIPs tailored for specific target analytes and incorporating them into cellulosic paper-based analytical devices, resulting in MIP-PADs that offer advantages such as affordability, portability, and disposability. Applications of MIP-PADs extend across environmental monitoring, food safety, and biomedical analysis, demonstrating exceptional selectivity and sensitivity toward diverse biomolecules, pathogens, and small molecules. Their affordability and user-friendly design make them particularly suitable for resource-limited settings. Lastly, the challenges and future prospects of MIP-PAD technologies are presented in the context of real-world applications.
分子印迹聚合物（MIPs）在生物传感中发挥着多功能且高选择性的作用，模拟生物分子受体并在复杂基质中与目标分析物有效相互作用。将MIPs与纸质分析装置（PADs）集成，能够实现分子印迹技术的快速、便捷且经济高效的应用。本文综述了MIP-PADs制备过程的进展，并探讨了它们的多种应用，重点介绍了其在现场检测中的实用性，所采用的检测机制包括比色法、荧光法、化学发光法、电化学法、光电化学法以及表面增强拉曼光谱法。制备过程包括合成针对特定目标分析物定制的MIPs，并将其整合到基于纤维素纸的分析装置中，从而得到具有价格实惠、便于携带和可一次性使用等优点的MIP-PADs。MIP-PADs的应用涵盖环境监测、食品安全和生物医学分析等领域，对各种生物分子、病原体和小分子表现出卓越的选择性和灵敏度。其价格实惠和用户友好的设计使其特别适用于资源有限的环境。最后，在实际应用的背景下介绍了MIP-PAD技术面临的挑战和未来前景。
