School of Pharmacy, Jiangsu University , Zhenjiang , China.
School of Traditional Chinese Medicine, China Pharmaceutical University , Nanjing , China.
Nat Prod Res. 2019 Nov;33(21):3095-3102. doi: 10.1080/14786419.2018.1519820. Epub 2018 Nov 23.
The rare secondary flavonol glycoside Sagittatoside A has much better in vivo bioactivities than epimedin A in Epimedii Folium. However, its current preparation methods are of low efficiency, with byproducts generated. The aim of this study was to establish a novel catalysis system for effective and convenient preparation of Sagittatoside A from epimedin A based on recyclable and integrated aqueous organic two-phase enzymatic hydrolysis. The system was consisted of propyl acetate and HAc-NaAc buffer (pH4.5) containing -dextranase/epimedin A, and the hydrolysis was performed at 60 °C for 1 h. Consequently, epimedin A was completely hydrolyzed to sagittatoside A, and 95.02% of the product was transferred into the organic phase. Moreover, 90% of its initial activity was retained after seven cycles of hydrolysis. Additionally, the procedure was simpler than conventional enzymatic hydrolysis. Collectively, the newly proposed strategy is an efficient and promising approach for the preparation of sagittatoside A in industrial application.
从淫羊藿中分离得到的黄酮醇糖苷型次级成分朝藿定 A 比朝藿定 B 具有更好的体内生物活性。但其目前的制备方法效率较低，且会产生副产物。本研究旨在建立一种新型的催化体系，通过可回收和集成的水相-有机相两相酶水解反应，从淫羊藿苷 A 有效且便捷地制备朝藿定 A。该体系由含有β-葡聚糖酶/淫羊藿苷 A 的乙酸丙酯和 HAc-NaAc 缓冲液（pH4.5）组成，在 60°C 下反应 1 h。结果表明，淫羊藿苷 A 完全水解为朝藿定 A，产物中有 95.02%转移到有机相中。此外，经过 7 次水解循环后，其初始活性保留了 90%。此外，该方法比传统的酶解法更简单。综上，该新策略为工业生产朝藿定 A 提供了一种高效、有前景的方法。
