Xu Feng-Juan, Sun E, Zhang Zhen-Hai, Cui Li, Jia Xiao-Bin
Zhongguo Zhong Yao Za Zhi. 2014 Jan;39(2):235-9.
To prepare sagittatoside B with epimedin B Hydrolyzed from cellulase. With the conversion ratio as the index, the effects of pH value, temperature, reaction time, dosage of enzyme and concentration of substrates on the conversion ratio were detected. L9 (3(4)) orthogonal design was adopted to optimize the preparation process. Hydrolyzed products were identified by MS, 1H-NMR, and 13C-NMR. The results showed that the optimum reaction conditions for the enzymatic hydrolysis were that the temperature was 50 degrees C, the reaction medium was pH 5.6 acetic acid-sodium acetate buffer solution, the concentration of substrates was 20 g x L(-1), the mass ratio between enzyme and substrate was 3: 5, and the relative molecular mass of the reaction product was 646.23. NMR data proved that the product was sagittatoside B. The process is simple and reliable under mild reaction conditions, thus suitable for industrial production.
以纤维素酶水解朝藿定B制备矢车菊苷B。以转化率为指标，考察pH值、温度、反应时间、酶用量和底物浓度对转化率的影响。采用L9(3(4))正交设计优化制备工艺。水解产物经质谱、1H-NMR和13C-NMR鉴定。结果表明，酶解的最佳反应条件为：温度50℃，反应介质为pH 5.6的乙酸-乙酸钠缓冲溶液，底物浓度为20 g·L(-1)，酶与底物质量比为3:5，反应产物相对分子质量为646.23。核磁共振数据证明产物为矢车菊苷B。该工艺反应条件温和，简单可靠，适合工业化生产。
