a Division of Endodontics and Restorative Dentistry, Department of Oral Functions , Kyushu Dental University , Kitakyushu , Japan.
b Research and Development Department , Nippon Shika Yakuhin Co., Ltd , Shimonoseki , Japan.
J Biomater Sci Polym Ed. 2019 Jan;30(1):49-63. doi: 10.1080/09205063.2018.1544474. Epub 2019 Jan 2.
Gelatin hydrogel sponges incorporating bioactive glasses (Gel-BG) were fabricated. We evaluated the characteristics of Gel-BG as scaffolds from the perspective of their mechanical properties and the formation of hydroxyapatite by the incorporation of bioactive glasses (BG). In addition, the Gel-BG degradation and the profile of fibroblast growth factor-2 (FGF-2) release from the Gel-BG were examined. Every Gel-BG showed an interconnected pore structure with the pore size range of 180-200 µm. The compression modulus of sponges incorporating BG increased. The time profiles of degradation for the 72-h crosslinked gelatin hydrogel sponges incorporating 10 wt% BG (Gel-BG(10)) and 50 wt% BG (Gel-BG(50)) were analogous to that of the 24-h crosslinked gelatin hydrogel sponge without BG (Gel-BG(0)). In measuring the release of FGF-2 from Gel-BG, the Gel-BG(10) and Gel-BG(50) showed almost analogous 100% cumulative release within 28 days in vivo. Additionally, a bioactivity evaluation showed that the presence of gelatin does not affect the in vitro bioactivity of Gel-BG. These sponges showed mechanical and chemical functionality as scaffolds, featuring both the controlled release of FGF-2 and the induction of hydroxyapatite crystallization.
制备了掺入生物活性玻璃（Gel-BG）的明胶水凝胶海绵。我们从机械性能和掺入生物活性玻璃（BG）形成羟基磷灰石的角度评估了 Gel-BG 作为支架的特性。此外，还研究了 Gel-BG 的降解以及从 Gel-BG 释放纤维细胞生长因子-2（FGF-2）的情况。每个 Gel-BG 都具有相互连接的孔结构，孔径范围为 180-200μm。掺入 BG 的海绵的压缩模量增加。72 小时交联的明胶水凝胶海绵掺入 10wt%BG（Gel-BG(10)）和 50wt%BG（Gel-BG(50)）的降解时间曲线与无 BG 的 24 小时交联的明胶水凝胶海绵（Gel-BG(0)）相似。在测量从 Gel-BG 释放 FGF-2 时，Gel-BG(10)和 Gel-BG(50)在体内 28 天内几乎以 100%的累积释放率表现出相似的结果。此外，生物活性评价表明明胶的存在并不影响 Gel-BG 的体外生物活性。这些海绵具有作为支架的机械和化学功能，其特点是 FGF-2 的控制释放和羟基磷灰石结晶的诱导。
