Washio Ayako, Kérourédan Olivia, Tabata Yasuhiko, Kokabu Shoichiro, Kitamura Chiaki
Division of Endodontics and Restorative Dentistry, Department of Oral Functions, Kyushu Dental University, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu 803-8580, Japan.
National Institute of Health and Medical Research (INSERM), U1026 BIOTIS, University of Bordeaux, 146 Rue Léo Saignat, 33076 Bordeaux, France.
J Funct Biomater. 2023 Dec 18;14(12):568. doi: 10.3390/jfb14120568.
Ideal regeneration of hard tissue and dental pulp has been reported with the use of a combination of bioactive glass and basic fibroblast growth factor (bFGF). However, no previous study has investigated the molecular mechanisms underlying the processes induced by this combination in dental pulp cells. This study aimed to examine the cellular phenotype and transcriptional changes induced by the combination of bioactive glass solution (BG) and bFGF in dental pulp cells using phase-contrast microscopy, a cell counting kit-8 assay, alkaline phosphatase staining, and RNA sequence analysis. bFGF induced elongation of the cell process and increased the number of cells. Whereas BG did not increase ALP activity, it induced extracellular matrix-related genes in the dental pulp. In addition, the combination of BG and bFGF induces gliogenesis-related genes in the nervous system. This is to say, bFGF increased the viability of dental pulp cells, bioactive glass induced odontogenesis, and a dual stimulation with bioactive glass and bFGF induced the wound healing of the nerve system in the dental pulp. Taken together, bioactive glass and bFGF may be useful for the regeneration of the dentin-pulp complex.
据报道，使用生物活性玻璃和碱性成纤维细胞生长因子（bFGF）的组合可实现硬组织和牙髓的理想再生。然而，此前尚无研究探讨这种组合在牙髓细胞中所诱导过程的分子机制。本研究旨在使用相差显微镜、细胞计数试剂盒-8检测、碱性磷酸酶染色和RNA序列分析，研究生物活性玻璃溶液（BG）和bFGF的组合在牙髓细胞中诱导的细胞表型和转录变化。bFGF诱导细胞突起伸长并增加细胞数量。虽然BG没有增加碱性磷酸酶活性，但它诱导了牙髓中与细胞外基质相关的基因。此外，BG和bFGF的组合诱导了神经系统中与神经胶质生成相关的基因。也就是说，bFGF增加了牙髓细胞的活力，生物活性玻璃诱导了牙本质生成，生物活性玻璃和bFGF的双重刺激诱导了牙髓中神经系统的伤口愈合。综上所述，生物活性玻璃和bFGF可能对牙本质-牙髓复合体的再生有用。
