Sadeghian Aida, Kharaziha Mahshid, Khoroushi Maryam
Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Int J Biol Macromol. 2023 Apr 15;234:123771. doi: 10.1016/j.ijbiomac.2023.123771. Epub 2023 Feb 20.
The study aims to develop a novel dentin extracellular matrix (dECM) loaded gelatin methacrylate (GelMA)-5 wt% bioactive glass (BG) (Gel-BG) hydrogel for dental pulp regeneration. We investigate the role of dECM content (2.5, 5, and 10 wt%) on the physicochemical characteristics and biological responses of Gel-BG hydrogel in contact with stem cells isolated from human exfoliated deciduous teeth (SHED). Results showed that the compressive strength of Gel-BG/dECM hydrogel significantly enhanced from 18.9 ± 0.5 kPa (at Gel-BG) to 79.8 ± 3.0 kPa after incorporation of 10 wt% dECM. Moreover, we found that in vitro bioactivity of Gel-BG improved and the degradation rate and swelling ratio reduced with increasing dECM content. The hybrid hydrogels also revealed effectual biocompatibility, >138 % cell viability after 7 days of culture; where Gel-BG/5%dECM was most suitable. In addition, the incorporation of 5 wt% dECM within Gel-BG considerably improved alkaline phosphatase (ALP) activity and osteogenic differentiation of SHED cells. Taken together, the novel bioengineered Gel-BG/dECM hydrogels having appropriate bioactivity, degradation rate, osteoconductive and mechanical properties represent the potential applications for clinical practice in the future.
该研究旨在开发一种用于牙髓再生的新型负载牙本质细胞外基质（dECM）的甲基丙烯酸明胶（GelMA）-5 wt%生物活性玻璃（BG）（Gel-BG）水凝胶。我们研究了dECM含量（2.5、5和10 wt%）对与从人脱落乳牙分离的干细胞（SHED）接触的Gel-BG水凝胶的物理化学特性和生物学反应的作用。结果表明，加入10 wt%的dECM后，Gel-BG/dECM水凝胶的抗压强度从18.9±0.5 kPa（Gel-BG时）显著提高到79.8±3.0 kPa。此外，我们发现随着dECM含量的增加，Gel-BG的体外生物活性提高，降解率和溶胀率降低。混合水凝胶还显示出有效的生物相容性，培养7天后细胞活力>138%；其中Gel-BG/5%dECM最为合适。此外，在Gel-BG中加入5 wt%的dECM显著提高了SHED细胞的碱性磷酸酶（ALP）活性和成骨分化。综上所述，具有适当生物活性、降解率、骨传导性和机械性能的新型生物工程Gel-BG/dECM水凝胶代表了未来临床实践中的潜在应用。
