Moodley Odeshnee, Sun Yue, Sossah Frederick Leo, Kakishima Makoto, Pavlov Igor N, Li Yu, Wang Qi
Engineering Research Centre of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, 130118, Jilin Province, China.
South African Department of Agriculture, Forestry and Fisheries (DAFF), Pretoria, 0001, South Africa.
Bull Environ Contam Toxicol. 2019 Feb;102(2):268-274. doi: 10.1007/s00128-018-2496-0. Epub 2018 Nov 23.
Alternaria oxytropis is an endophytic fungus of locoweeds that synthesizes swainsonine toxin. In this work, we evaluated the effect of A. oxytropis on soybean seedlings and quantified swainsonine in different culture conditions. Soybean (Glycine max) seeds were co-cultured with A. oxytropis (at different concentrations of mycelial suspensions) in agar media and soil culture, and swainsonine was assayed using LC-MS/MS. The results showed evidence that A. oxytropis infected soybean seedlings produced detectable swainsonine in agar culture while the toxin was undetectable or below the detection limit (0.006% of swainsonine dry weight) in soil media even at higher concentrations of the fungus. These results suggest that swainsonine detection is highly dependent on culture conditions and that soybeans co-cultured with A. oxytropis in soil could potentially be used to limit toxin production.
棘豆链格孢是一种能合成苦马豆素毒素的疯草内生真菌。在本研究中，我们评估了棘豆链格孢对大豆幼苗的影响，并对不同培养条件下的苦马豆素进行了定量分析。大豆（Glycine max）种子在琼脂培养基和土壤培养中与棘豆链格孢（不同浓度的菌丝悬浮液）共培养，并用液相色谱-串联质谱法测定苦马豆素。结果表明，在琼脂培养中，受棘豆链格孢感染的大豆幼苗能产生可检测到的苦马豆素，而在土壤培养基中，即使真菌浓度较高，该毒素也无法检测到或低于检测限（苦马豆素干重的0.006%）。这些结果表明，苦马豆素的检测高度依赖于培养条件，并且在土壤中与棘豆链格孢共培养的大豆可能有助于限制毒素的产生。
