School of Pharmacy, Lanzhou University, West Donggang Road No. 199, Lanzhou 730000, PR China.
Key Laboratory of New Animal Drug Project, Gansu Province/Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs/Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agriculture Sciences, No. 335, Qilihe District, Lanzhou 730050, PR China.
FEMS Microbiol Lett. 2021 May 11;368(8). doi: 10.1093/femsle/fnab047.
Swainsonine, an indolizidine alkaloid, is a promising anti-tumorigenic compound. Biological production of swainsonine was prospective, but the low swainsonine yield of wild type Alternaria oxytropis limited its production on a large scale. In present work, a stable A. oxytropis mutant UO1 with swanisonine yield of 14.84% higher than the wild-type strain was successfully obtained after heavy-ion irradiation. The A. oxytropis mutant UO1 and original wild-type strain were futher evaluated for SW concentrations under different factors. Results showed that the optimum culture temperature was 25°C. The optimum initial medium pH was 6.5 and the optimum inoculum size was 2 mL per 200 mL. Addition of the biosynthetic precursor L-pipecolic acids and L-lysine appropriately could increase the SW synthesis. These findings provided a theoretical basis and scientific data for the industrial production of swainsonine.
苦马豆素是一种吲哚里西啶生物碱，具有抗肿瘤的作用。苦马豆素具有潜在的生物生产价值，但野生型 Oxytropis glabra 产苦马豆素的产量低，限制了其大规模生产。本研究采用重离子辐照诱变的方法，成功获得了苦马豆素产量比野生型菌株提高 14.84%的稳定突变株 UO1。进一步研究不同因素对突变株 UO1 和原始野生型菌株 SW 浓度的影响。结果表明，最适培养温度为 25℃；最适初始培养基 pH 值为 6.5，最适接种量为每 200 mL 2 mL。适当添加生物合成前体 L-高精氨酸和 L-赖氨酸可以增加 SW 的合成。这些发现为苦马豆素的工业生产提供了理论依据和科学数据。
