Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, People's Republic of China; College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, People's Republic of China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, People's Republic of China; College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, People's Republic of China.
Bioresour Technol. 2019 Feb;273:515-520. doi: 10.1016/j.biortech.2018.11.061. Epub 2018 Nov 16.
Bioprocess for successive bio-production of glycolic acid (GA) from ethylene glycol (EG) using Gluconobacter oxydans is hindered by strong end-product inhibitory effect. Based on the model of compressed oxygen supplied-sealed stirred tank reactor (COS-SSTR), we developed a new system by attaching an ultrafiltration instrument and electrodialysis cell to in-situ separate GA, including conductivity meter to control automatic EG feeding. The combined bioprocess was therefore set up as compressed oxygen supplied cell catalysis-ultrafiltration-electrodialysis (COS-CUE). In comparison with the conventional resin and electrodialysis separation process, this device simplified the whole bioprocess. We realized the potential of combined bioprocess for producing GA without EG through continuous/semi-continuous 'one-step' process. Finally, 288.4 g GA was obtained at the yield of 96.5% and average productivity of 4.0 g/L/h in 72 h, with an increment of 148.8% and 20.9% in production compared with batch and cell-recycling fermentation.
利用氧化葡萄糖酸杆菌（Gluconobacter oxydans）从乙二醇（EG）连续生物生产乙醇酸（GA）的生物工艺受到强末端产物抑制作用的阻碍。基于供氧密封搅拌罐反应器（COS-SSTR）模型，我们通过附加超滤仪器和电渗析单元开发了一种新系统，用于原位分离 GA，包括电导率计来控制自动 EG 进料。因此，组合生物工艺被设置为供氧细胞催化-超滤-电渗析（COS-CUE）。与传统的树脂和电渗析分离工艺相比，该设备简化了整个生物工艺。我们通过连续/半连续“一步法”实现了无 EG 进行 GA 生产的联合生物工艺的潜力。最终，在 72 小时内获得了 288.4g 的 GA，产率为 96.5%，平均生产率为 4.0g/L/h，与分批和细胞循环发酵相比，产量增加了 148.8%，生产率提高了 20.9%。
