DNA修复基因XRCC3和XRCC1在2型糖尿病及糖尿病肾病易感性中的作用。
Role of DNA repair genes XRCC3 and XRCC1 in predisposition to type 2 diabetes mellitus and diabetic nephropathy.
作者信息
Yesil-Devecioglu Tugce, Dayan Akın, Demirtunc Refik, Sardas Semra
机构信息
Marmara University, Faculty of Pharmacy, Department of Pharmaceutical Toxicology, Istanbul, Turkey.
Health Sciences University, Haydarpasa Numune Training and Research Hospital, Diabetology Outpatient Department, Istanbul, Turkey.
出版信息
Endocrinol Diabetes Nutr (Engl Ed). 2019 Feb;66(2):90-98. doi: 10.1016/j.endinu.2018.08.010. Epub 2018 Nov 22.
INTRODUCTION
Increasing number of experimental and clinical studies suggest a strong relationship between hyperglycemia, oxidative stress, DNA damage and diabetic nephropathy (DN). Also, epidemiologic studies remark an enhanced risk of cancer with type 2 diabetes. This research aims to assess whether the X-ray cross complementing group 3 (XRCC3) gene T241M polymorphism (rs861539) and X-ray cross complementing group 1 (XRCC1) gene A399G polymorphism (rs25487) are related with predisposition to type 2 diabetes mellitus (T2DM) and to diabetic nephropathy in Turkish population.
MATERIALS AND METHODS
Polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) was performed to identify the distribution of genotypes and frequency of alleles of T241M polymorphism of the XRCC3 gene (XRCC3 T241M) and A399G polymorphism of the XRCC1 gene (XRCC1 A399G). The study population included 238 subjects residing in Istanbul, Turkey; 116 with T2DM, 50 with DN and 72 with normal glucose metabolism.
RESULTS AND CONCLUSION
Polymorphic Gln allele of XRCC1 gene was significantly related with T2DM and DN (OR 3.09, 95% CI 1.14-8.40 and OR 3.29 95% CI 1.23-8.80, respectively) however, there was no statistical association of XRCC3 T241M with T2DM or DN. The results of this study suggest that XRCC1 399Gln polymorphism is related with an increased susceptibility to T2DM and DN in the studied Turkish population.
引言
越来越多的实验和临床研究表明，高血糖、氧化应激、DNA损伤与糖尿病肾病（DN）之间存在密切关系。此外，流行病学研究指出2型糖尿病患者患癌症的风险增加。本研究旨在评估X射线交叉互补组3（XRCC3）基因T241M多态性（rs861539）和X射线交叉互补组1（XRCC1）基因A399G多态性（rs25487）是否与土耳其人群中2型糖尿病（T2DM）及糖尿病肾病的易感性相关。
材料与方法
采用基于聚合酶链反应的限制性片段长度多态性分析（PCR-RFLP）来确定XRCC3基因T241M多态性（XRCC3 T241M）和XRCC1基因A399G多态性（XRCC1 A399G）的基因型分布及等位基因频率。研究人群包括居住在土耳其伊斯坦布尔的238名受试者；116例T2DM患者、50例DN患者和72例葡萄糖代谢正常者。
结果与结论
XRCC1基因的多态性Gln等位基因与T2DM和DN显著相关（OR分别为3.09，95%CI为1.14 - 8.40；OR为3.29，95%CI为1.23 - 8.80），然而，XRCC3 T241M与T2DM或DN之间无统计学关联。本研究结果表明，在受试的土耳其人群中，XRCC1 399Gln多态性与T2DM和DN的易感性增加有关。
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